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Extracellular polymeric substances of the marine fouling diatom Amphora rostrata Wm. Sm.

DRS at CSIR-National Institute of Oceanography

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Title Extracellular polymeric substances of the marine fouling diatom Amphora rostrata Wm. Sm.
 
Creator Khandeparker, R.
Bhosle, N.B.
 
Subject fouling organisms
films
isolating mechanisms
water purification
growth
chemical extraction
Amphora rostrata
 
Description Amphora rostrata was grown under continuous illumination at 27 degrees C in batch cultures using f/2 medium. Cell biomass (measured as chlorophyll a and cell counts) reached a maximum on day 7. Thereafter, cell biomass as chl a showed a small decrease. Planktonic ('free') and biofilm extracellular polymeric substances (EPS) from the adherent cells of A. rostrata were studied. Both types of EPS were produced during the logarithmic phase of growth. However, production was higher during the stationary growth phase. Enhanced EPS production was associated with nutrient deficient conditions. Planktonic and biofilm EPS were purified by gel filtration using Sephadex G-200 and ion exchange chromatography using DEAE-cellulose. Both polymers showed the presence of a single peak. Capillary gas chromatographic analysis of both planktonic and biofilm EPS showed that fucose (36.7%) and galactose (27.6%) were the most abundant monosaccharides, with small quantities of rhamnose, xylose, arabinose, mannose and glucose. Other chemical analysis showed the presence of sulphate, uronic acids, hexoamines, pyruvate and proteins in both the planktonic and biofilm EPS. Uronic acid, pyruvate and sulphate together were found to contribute approximately 50 to 60% (W/W) to the EPS of A. rostrata. Such a high content of non-sugar components indicates their importance to the diatom in metal binding, desiccation prevention and flexibility
 
Date 2009-01-07T09:35:04Z
2009-01-07T09:35:04Z
2001
 
Type Journal Article
 
Identifier Biofouling, Vol.17; 117-127p.
http://drs.nio.org/drs/handle/2264/1514
 
Language en
 
Rights Copyright [2001]. All efforts have been made to respect the copyright to the best of our knowledge. Inadvertent omissions, if brought to our notice, stand for correction and withdrawal of document from this repository.
 
Publisher Taylor and Francis