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Detection of luciferase gene sequences in nonluminescent bacteria from the Chesapeake Bay

DRS at CSIR-National Institute of Oceanography

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Title Detection of luciferase gene sequences in nonluminescent bacteria from the Chesapeake Bay
 
Creator Ramaiah, N.
Chun, J.
Ravel, J.
Straube, W.L.
Hill, R.T.
Colwell, R.R.
 
Subject microorganisms
genes
chitin
strains
luminescence
genotypes
Bacteria
 
Description A 745-bp luxA fragment was amplified from Vibrio harveyi (UM 1503), radiolabeled, and used as a probe to detect and quantify luxA genotypes in culturable bacterial populations from the Chesapeake Bay. DNA samples from 53 reference strains were also examined for this gene. The luxA-positive bacteria comprised from 0-6% of the culturable heterotrophic bacterial community in samples from the Bay. Only those reference strains known to be luminescent contained the luxA gene, as indicated by PCR. Results in all cases were confirmed by PCR of DNA extracts and Southern hybridization analyses, using an internal probe for confirmation of luxA amplification products. Sequence analysis of luxA genes from three nonluminescent bacteria isolated from the Chesapeake Bay indicated little or no differences when compared with luxA sequences from known marine luminescent bacterial species. These three Chesapeake Bay strains and other luxA-positive strains were tested with a luminometer and confirmed to be nonluminescent. All of over 7800 bacterial colonies enumerated during this study from Chesapeake Bay samples were non-visibly luminescent. The results indicate that luxA-positive bacteria isolated from the Chesapeake Bay are not generally luminescent on phenotypic examination, implying that gene probe techniques are required for examining luxA gene distribution in microbial populations present in environmental samples.
 
Date 2009-01-09T07:41:50Z
2009-01-09T07:41:50Z
2000
 
Type Journal Article
 
Identifier FEMS Microbiology Ecology, Vol.33; 27-34p.
http://drs.nio.org/drs/handle/2264/1621
 
Language en
 
Rights Copyright [2000]. All efforts have been made to respect the copyright to the best of our knowledge. Inadvertent omissions, if brought to our notice, stand for correction and withdrawal of document from this repository.
 
Publisher FEMS