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Lignin-modifying enzymes of Flavodon flavus, a basidiomycete isolated from a coastal marine environment

DRS at CSIR-National Institute of Oceanography

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Title Lignin-modifying enzymes of Flavodon flavus, a basidiomycete isolated from a coastal marine environment
 
Creator Raghukumar, C.
DeSouza, T.M.
Thorn, R.G.
Reddy, C.A.
 
Subject microorganisms
biochemical phenomena
biodegradation
carbon cycle
enzymes
enzymatic activity
isoenzymes
nitrogen cycle
Flavodon flavus
 
Description A basidiomycetous fungus Flavodon flavus (Klotzsch) Ryvarden (strain 312), isolated from decaying sea grass from a coral lagoon off the west coast of India, mineralized nearly 24% of sup(14)C-labeled synthetic lignin to sup(14)CO sub(2) in 24 days. When grown in low-nitrogen medium (2.4 mM N) this fungus produced three major classes of extracellular lignin-modifying enzymes (LMEs): manganese-dependent peroxidase (MNP), lignin peroxidase (LIP), and laccase. Low MNP and laccase activities were seen in high-nitrogen medium (24 mM N), but no LIP activity was seen. In media containing lignocellulosic substrates such as pine, poplar, or sugarcane bagasse as the sole source of carbon and nitrogen, relatively hgih MNP and moderate levels of laccases were seen, but LIP production either was not seen or was minimal. LME rpoduction was also seen in media prepared with artificial seawater. Fast rpotein liquid chromatography and isoelectric focusing resolved LMEs into four isozymes each of MNP and LIP, while laccase isozymes were resolved into two groups, one group containing seven isozymes (pIs 4 to 6) and the other group containing at least three isozymes (pIs less than 3). The molecular masses of the different isozymes were 43 to 99 kDa for MNP, 40 and 41.5 kDa for LIP, and 43 and 99 kDa for laccase. F. flavus showed effective degradation of various dye pollutants of various dye pollutants in media prepared with or without artificial seawater. This is the first report on the production of all three major classes of LMEs by F. flavus and points to the bioremediation potential of this organism in terrestrial as well as marine environments
 
Date 2009-01-10T11:51:45Z
2009-01-10T11:51:45Z
1999
 
Type Journal Article
 
Identifier Applied and Environmental Microbiology, Vol.65; 2103-2111p.
http://drs.nio.org/drs/handle/2264/1843
 
Language en
 
Rights Copyright [1999]. All efforts have been made to respect the copyright to the best of our knowledge. Inadvertent omissions, if brought to our notice, stand for correction and withdrawal of document from this repository.
 
Publisher American Society for Microbiology