Record Details

Cryoperservation of Balanus amphitrite nauplii

DRS at CSIR-National Institute of Oceanography

View Archive Info
 
 
Field Value
 
Title Cryoperservation of Balanus amphitrite nauplii
 
Creator Anil, A.C.
Tulaskar, A.S.
Khandeparker, D.C.
Wagh, A.B.
 
Subject crustacean larvae
nauplii
survival
freezing storage
cryobiology
temperature tolerance
cooling
rearing
metamorphosis
Balanus amphitrite
 
Description The possibility of cryopreserving B. amphitrite (Cirripedia; thoracica) nauplii is explored. The effects on the postthaw survival of a range of cryobiological variables are reported; three different cryoprotectants (ethylene glycol, EG; dimethyl sulfoxide, Me sub(2)SO; and glycerol, GL), the influence of the final temperature (-20, -30, -40, and -50 degrees C), and the effect of transferring to liquid nitrogen (-196 degrees C) were studied. The larvae showed increasing susceptibility to cryoprotectant concentration. Equilibration of larvae for 15 min at 5 degrees C with each of the cryoprotectants revealed and GL was the least toxic. EG at 3 to 4 M did not cause marked damage as the equilibration period was extended up to 2 h. This was not true with Me sub(2)SO and GL. The postthaw survival rate decreased as the final temperature was reduced, and none of the larvae revived after thawing from -50 degrees C. The postthaw survival with 2 mol/liter EG as the cryoprotectant ranged from 86% after cooling to -20 degrees C to 56% after cooling to -40 degrees C. The larvae that were transferred to liquid nitrogen from different temperatures revealed that the greatest survival (36%) was achieved when the larvae were transferred from -40 to -196 degrees C. When the larvae reared after cooling to -40 degrees C and then transferring to liquid nitrogen, the rate of subsequent metamorphosis was 30% in the case of EG at 3M. Me sub(2)SO and GL gave results that were inferior in comparison to those obtained with EG
 
Date 2009-01-12T08:40:14Z
2009-01-12T08:40:14Z
1997
 
Type Journal Article
 
Identifier Cryobiology, Vol.34; 131-140p.
http://drs.nio.org/drs/handle/2264/1984
 
Language en
 
Rights Copyright [1997]. All efforts have been made to respect the copyright to the best of our knowledge. Inadvertent omissions, if brought to our notice, stand for correction and withdrawal of document from this repository.
 
Publisher Academic Press