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A study on the effects of some laboratory-derived genetic mutations on biofilm formation by Listeria monocytogenes

DRS at CSIR-National Institute of Oceanography

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Title A study on the effects of some laboratory-derived genetic mutations on biofilm formation by Listeria monocytogenes
 
Creator Kumar, S.
Parvathi, A.
George, J.
Krohne, G.
Karunasagar, Indrani
Karunasagar, Iddya
 
Subject laboratory culture
Listeria monocytogenes
biofilms
microorganisms
 
Description Biofilms formed by the human pathogen Listeria monocytogenes in food-processing environments can be a potential source of contamination. In this study, we investigated the ability of L. monocytogenes wild type and its laboratory-derived isogenic mutants in cwhA, prfA, agrA, flaA, degU, ami and sigB to adhere to and form biofilms on abiotic surfaces. The results suggest that inactivation of the two component regulatory system degU completely abolished biofilm formation, while inactivation of the flagellar gene flaA, two component response regulator agrA and the autolysin-adhesin gene ami lead to severe impairment of initial attachment and the subsequent development of a mature biofilm by L. monocytogenes. Mutants in the global regulator of virulence prfA and the alternative sigma factor sigB were unaffected and formed biofilms similar to wild type L. monocytogenes
 
Date 2009-03-30T10:16:37Z
2009-03-30T10:16:37Z
2009
 
Type Journal Article
 
Identifier World Journal of Microbiology and Biotechnology, vol.25(3); 527-531
http://drs.nio.org/drs/handle/2264/2604
 
Language en
 
Rights An edited version of this paper was published by Springer. This paper is for R & D pupose and Copyright [2009] Springer.
 
Publisher Springer