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Bacterial bioluminescence in marine pollution assessment

DRS at CSIR-National Institute of Oceanography

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Title Bacterial bioluminescence in marine pollution assessment
 
Creator Ramaiah, N.
Chandramohan, D.
 
Subject bioluminescence
microorganisms
growth
marine pollution
pollution detection
comparative studies
Vibrio harveyi
Vibrio fischeri
Photobacterium leiognathi
 
Description Warm water marine luminous bacterial species, particularly Vibrio harveyi, V. fischeri and Photobacterium leiognathi, are easy to isolate, maintain and handle in the laboratories without strict temperature requirements, which is an important advantage in the tropics. With evaluation of their response to texicants as the main objective, detailed studies on their physiology and optimisation of culture conditions were carried out. Quantitative output of luminescence from many strains of these species was monitored upon additions of micro and nanomolar concentrations of numerous toxic (heavy metals, pesticides, phenolic compounds, amines, aldehydes, organic solvents, commercial detergents, crude petroleum extracts) and nontoxic (essential salts, sugars, amino acids) substances. A very short time (300 sec) bioassay method was devised and on the basis of these and a few other studies, it was discernible that live cells of these luminous prokaryotes are helpful in reliable detection of even the picomolar concentrations of toxic materials. Results of this study highlight the possibility of employing these bacteria in a quick prescreening of the marine environment in the pollution monitoring programmes and other advantages with them
 
Date 2009-05-05T04:40:06Z
2009-05-05T04:40:06Z
1994
 
Type Conference Article
 
Identifier Ocean technology: Perspectives, Ed. by: Sushilkumar; Agadi, V.V.; Das, V.K.; Desai, B.N. 967-980p.
http://drs.nio.org/drs/handle/2264/2654
 
Language en
 
Rights Copyright [1994]. All efforts have been made to respect the copyright to the best of our knowledge. Inadvertent omissions, if brought to our notice, stand for correction and withdrawal of document from this repository.
 
Publisher Publ. and Inf. Dir., New Delhi, India