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Gynogenetic induction in marine bivalve molluscs for improvement of stocks: Standardization of some important factors

DRS at CSIR-National Institute of Oceanography

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Title Gynogenetic induction in marine bivalve molluscs for improvement of stocks: Standardization of some important factors
 
Creator Goswami, U.
 
Subject marine molluscs
stock assessment
genes
sperm
standardization
 
Description Experiments conducted for standardizing sperm density for artificial insemination of Chlamys mobilis prior to inducing gynogenesis by ultra-violet rays irradiated sperms showed that 10:1 ratio of sperms to eggs should be maintained for getting maximum rate of fertilization and the normal development of the veliger larvae. The optimum quantity and density of sperm suspension recommended for irradiation exposure experiments in 10 ml capacity glass petri dish was 0.5 ml of stock sperm suspension with 20 x 10 super(6) per ml concentration so as to effectively inseminate 2000-2500 eggs in 50 ml of seawater. The results of the preliminary gynogenetic induction experiments performed ascertained the reliability of these parameter limits. In controls, it showed 100% rate of fertilization and 95.00% normality in the form of morulae after 6 hours of insemination and 98.05% metamorphosis and 96.12% normality in the form of normal D-shaped larvae. 24 hours after insemination. The lower values of fertilization, metamorphosis and normality in the other groups were due to the insemination of eggs with the ultra-violet ray irradiated sperms. The gynogenetic haploid larvae with 16 chromosomes were induced
 
Date 2009-05-06T09:30:34Z
2009-05-06T09:30:34Z
1993
 
Type Conference Article
 
Identifier Proceedings of the National Seminar on Aquaculture Development in India- Problems and Prospects, 27-29 November, 1990, Ed. by: Natarajan, P.; Jayaprakas, V. 263-268p.
http://drs.nio.org/drs/handle/2264/2741
 
Language en
 
Rights Copyright [1993]. All efforts have been made to respect the copyright to the best of our knowledge. Inadvertent omissions, if brought to our notice, stand for correction and withdrawal of document from this repository.
 
Publisher University of Kerala, Thiruvananthapuram