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Biochemical and molecular characterizaion of Bacillus pumilus isolated from coastal environment in Cochin, India

DRS at CSIR-National Institute of Oceanography

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Title Biochemical and molecular characterizaion of Bacillus pumilus isolated from coastal environment in Cochin, India
 
Creator Parvathi, A.
Krishna, K.
Jose, J.
Joseph, N.
Nair, S.
 
Subject Bacillus pumilus
microorganisms
polymerase chain reaction
biochemical analysis
molecular structure
coastal environment
 
Description Bacillus species constitute a diverse group of bacteria widely distributed in soil and the aquatic environment. In this study, Bacillus strains isolated from the coastal environment of Cochin, India were identified by detailed conventional biochemical methods, fatty acid methyl ester (FAME) analysis and partial 16S rDNA sequencing. Analysis of the data revealed that Bacillus pumilus was the most predominant species in the region under study followed by B. cereus and B. sphaericus. The B. pumilus isolates were further characterized by arbitrarily primed PCR (AP-PCR), antibiotic sensitivity profiling and PCR screening for known toxin genes associated with Bacillus spp. All B. pumilus isolates were biochemically identical, exhibited high protease and lipase activity and uniformly sensitive to antibiotics tested in this study. One strain of B. pumilus harboured cereulide synthetase gene cesB of B. cereus which was indistinguishable from rest of the isolates biochemically and by AP-PCR. This study reports, for the first time, the presence of the emetic toxin gene cesB in B. pumilus
 
Date 2009-06-23T11:18:30Z
2009-06-23T11:18:30Z
2009
 
Type Journal Article
 
Identifier Brazilian Journal of Microbiology, vol.40; 269-275
http://drs.nio.org/drs/handle/2264/3364
 
Language en
 
Rights Copyright [2009]. All efforts have been made to respect the copyright to the best of our
knowledge. Inadvertent omissions, if brought to our notice, stand for correction and withdrawal of document from this repository.
 
Publisher Sociedade Brasileira de Microbiologia