Record Details


Field	Value

Title	Prevalence and heterogeneity of Hemolysin gene vhh among hatchery isolates of Vibrio harveyi in India

Creator	Parvathi, A. George, J. Kumar, S.

Subject	bacterial diseases polymerase chain reaction Vibrio harveyi microorganisms

Description	Vibrio harveyi, pathogenic to fish, harbor a hemolysin gene vhh, the homologues of which are found in many species of the Genus Vibrio. It is investigated that the prevalence of vhh gene among V. harveyi isolated from Penaeus monodon hatcheries in India by polymerase chain reaction (PCR). The vhh was detected in 67 of the 70 V. harveyi isolates tested in this study using different combinations of PCR primers. A variant vhh gene detected in a minority of strains was cloned, sequenced, and the recombinant protein was expressed in Escherichia coli. The deduced amino acid sequence of the cloned gene was 86% similar to the previously reported amino acid sequences of VHH. The results of this study suggest that though V. harveyi strains invariably harbor vhh, the sequence variants of the hemolysin gene exist that may impede their detection by PCR.

Date	2009-06-26T04:15:03Z 2009-06-26T04:15:03Z 2009

Type	Journal Article

Identifier	Current Microbiology, vol.59; 42-47 http://drs.nio.org/drs/handle/2264/3366

Language	en

Rights	An edited version of this paper was published by Springer. This paper is for R & D purpose and Copyright [2009] Springer.

Publisher	Springer

ICAR Research Data Repository for Knowledge Management