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Effects and interactions of medium components on laccase from a marine-derived fungus using response surface methodology

DRS at CSIR-National Institute of Oceanography

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Title Effects and interactions of medium components on laccase from a marine-derived fungus using response surface methodology
 
Creator DeSouza-Ticlo, D.
Garg, S.
Raghukumar, C.
 
Subject marine-derived basidiomycete
laccase
Plackett Burman Design
Central Composite Design
 
Description The effects of various synthetic medium components and their interactions with each other ultimately impact laccase production in fungi. This was studied using a laccase-hyper-producing marine-derived basidiomycete, Cerrena unicolor MTCC 5159. Inducible laccases were produced in the idiophase only after addition of an inducer such as CuSO sub(4). Concentration of carbon and nitrogen acted antagonistically with respect to laccase production. A combination of low nitrogen and high carbon concentration favored both biomass and laccase production. The most favorable combination resulted in 917 U L sup(-1) of laccase. After sufficient growth had occurred, addition of a surfactant such as Tween 80 positively impacted biomass and increased the laccase activity to around 1,300 U L sup(-1). Increasing the surface to volume ratio of the culture vessel further increased its activity to almost 2,000 U L sup(-1)
 
Date 2009-12-08T07:14:50Z
2009-12-08T07:14:50Z
2009
 
Type Journal Article
 
Identifier Marine Drugs, vol.7(4); 672-688
http://drs.nio.org/drs/handle/2264/3466
 
Language en
 
Publisher Molecular Diversity Preservation International