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Detection of DNA damage in fish Oreochromis mossambicus induced by co-exposure to phenanthrene and nitrite by ESI-MS/MS

DRS at CSIR-National Institute of Oceanography

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Title Detection of DNA damage in fish Oreochromis mossambicus induced by co-exposure to phenanthrene and nitrite by ESI-MS/MS
 
Creator Wahidullah, S.
Rajamanickam, Y.R.
 
Subject brackishwater fish
DNA damage
aromatic hydrocarbons
Oreochromis mossambicus
 
Description Mutagenic nitrated polycyclic aromatic hydrocarbons (nitro-PAHs) have been known to arise in the environment through direct emissions from combustion sources and nitration of PAHs, primarily in the atmosphere. In the marine environment, PAHs are one of the classic anthropogenic organic pollutants, while nitrite (NO sub(2) sup(-)) is produced naturally via various biological processes like imbalance in ditrification/denitrification or eutrophication and subsequent oxygen depletion from an oversupply of nutrients. In this paper, we report the formation of PAH-DNA adducts in fish contaminated with PAHs and exposed to NO sub(2) sup (-) in the ambient water. Electrospray ionization tandem mass spectrometric (ESI-MS/MS) analysis of the bile of the euryhaline fish Oreochromis mossambicus exposed simultaneously to field relevant sublethal concentrations of phenanthrene and NO sub(2) sup(-) and collision-induced dissociation of selected ions revealed the presence of DNA-PAH adducts. The present study indicates that, although several high sensitivity techniques have been developed for the analysis of PAH derived DNA adducts, MS/MS has emerged as a powerful tool in the detection and structure elucidation of DNA adducts.
 
Date 2010-02-16T07:40:46Z
2010-02-16T07:40:46Z
2010
 
Type Journal Article
 
Identifier Environmental Science and Pollution Research, vol.17(2); 441-452
no
http://drs.nio.org/drs/handle/2264/3546
 
Language en
 
Relation dnadetection.jpg
 
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Publisher Springer-Verlag