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Identification of upregulated immune-related genes in Vibrio harveyi challenged Penaeus monodon postlarvae

DRS at CSIR-National Institute of Oceanography

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Title Identification of upregulated immune-related genes in Vibrio harveyi challenged Penaeus monodon postlarvae
 
Creator Nayak, S.
Singh, S.K.
Ramaiah, N.
Sreepada, R.A.
 
Subject Penaeus monodon
Shrimp diseases
Vibrio harveyi
 
Description A subtracted cDNA library was constructed and analyzed to elucidate the response of Penaeus monodon postlarvae challenged with Vibrio harveyi. As many as 960 randomly selected cDNA fragments generated through suppression subtractive hybridization were single pass sequenced. Forty five genes and 20 hypothetical proteins were identified, a few being first reports from shrimps. The most abundant immune relevant genes were ferritin, hemocyanin, and TCTP (translationally controlled tumor protein) indicating their upregulation as also confirmed through qPCR. Post-infection qPCR analyses confirmed 2.04, 2.09, 3.28, 5.49, 6.47, and 11.63 fold rise respectively in ferritin, penaeidin, MnSOD, lysozyme, TCTP, and hemocyanin genes. These genes may be involved in the regulation of the host defense against V. harveyi
 
Date 2010-07-15T12:24:17Z
2010-07-15T12:24:17Z
2010
 
Type Journal Article
 
Identifier Fish & Shellfish Immunology, vol.29(3); 544-549
http://drs.nio.org/drs/handle/2264/3656
 
Language en
 
Rights An edited version of this paper was published by Elsevier. Copyright [2010] Elsevier
 
Publisher Elsevier