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Life history of a free-living marine nematode Daptonema normandicum reared in laboratory

DRS at CSIR-National Institute of Oceanography

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Title Life history of a free-living marine nematode Daptonema normandicum reared in laboratory
 
Creator Singh, R.
Ingole, B.S.
 
Subject Daptonema normandicum
meiobenthic
nematode
biodiversity
 
Description Life history of a free-living meiobenthic nematode Daptonema normandicum (DeMan, 1890) was studied in the laboratory. Live specimens were primarily collected from the sewage outlet site near the mouth of the Mandovi estuary, Goa. This species was the most dominant (>67%) among the meiobenthic nematodes. Vertically, nematode abundance was highest at the surface sediment and correlated with the organic carbon and sediment chlorophyll-a. Considering their dominance in the meiofauna, attempts were made to rear D. normandicum in laboratory. Salinity of the culture medium was maintained at 14 to 17 PSU (same as the collection site). All the culture experiments were conducted in semisolid nutrient agar media at 27 + or ? 2 degrees C temperature for 12 hr dark: 12 hr light conditions. The food consists primarily of an unidentified bacterium and mixed algae, but diatom and ciliates were also observed in culture. Females produced first batch of eggs at the age of 23 days. Gravid female normally carry 8-10 eggs. Embryonic development is completed in approx. 72 hr and entire life cycle (egg to adult) was completed in 22-24 days. Average size of juveniles at the hatching was 0.189 mm. Young individuals attains a maximum size of 1.23 mm (male) and 1.04 mm (female) in approx. 21-23 days. Growth, in terms of length was augmented upto 23rd day and ceased thereafter. The daily growth increment for the first 5 days was 0.01-0.04 mm which increased upto 0.05-0.08 mm d-1 during the maturation (10-18 days). Male : female ratio was 1:2. In this laboratory study, we provided information on the embryonic development, the life cycle and ecology. Our results demonstrated that D. normandicum can be reared successfully under the controlled conditions, suggesting possible use of this species in toxicological and aquaculture studies. The culture method described is very handy and can be applicable for rearing other meiobenthic species particularly the nematodes with comparable feeding habits
 
Date 2011-03-09T10:55:40Z
2011-03-09T10:55:40Z
2011
 
Type Journal Article
 
Identifier Journal of Environmental Biology, vol.32(2); 2011; 147-152
http://drs.nio.org/drs/handle/2264/3808
 
Language en
 
Rights Copyright [2011] Triveni Enterprises. All efforts have been made to respect the copyright to the best of our knowledge. Inadvertent omissions, if brought to our notice, stand for correction and withdrawal of document from this repository.
 
Publisher Triveni Enterprises