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Fungal community analysis in the deep-sea sediments of the central Indian Basin by culture-independent approach

DRS at CSIR-National Institute of Oceanography

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Title Fungal community analysis in the deep-sea sediments of the central Indian Basin by culture-independent approach
 
Creator Singh, P.
Raghukumar, C.
Verma, P.
Shouche, Y.
 
Subject sediments
Central Indian Basin
marine fungi
18S rRNA gene
 
Description Few studies have addressed the occurrence of fungi in deep-sea sediments, characterized by elevated hydrostatic pressure, low temperature, and fluctuating nutrient conditions. The diversity of fungi at three locations of the Central Indian Basin (CIB) at a depth of approx. 5,000 m using culture-independent approach is evaluated. Community DNA isolated from these sediments was amplified using universal and fungal-specific internal transcribed spacers and universal 18S rDNA primer pairs. A total of 39 fungal operational taxonomic units, with 32 distinct fungal taxa were recovered from 768 clones generated from 16 environmental clone libraries. The application of multiple primers enabled the recovery of eight sequences that appeared to be new. The majority of the recovered sequences belonged to diverse phylotypes of Ascomycota and Basidiomycota. Our results suggested the existence of cosmopolitan marine fungi in the sediments of CIB. This study further demonstrated that diversity of fungi varied spatially in the CIB. Individual primer set appeared to amplify different fungal taxa occasionally. This is the first report on culture-independent diversity of fungi from the Indian Ocean
 
Date 2011-04-15T07:47:12Z
2011-04-15T07:47:12Z
2011
 
Type Journal Article
 
Identifier Microbial Ecology, vol.61; 2011; 507-517
no
http://drs.nio.org/drs/handle/2264/3842
 
Language en
 
Relation Microb_Ecol_61_507.jpg
 
Rights An edited version of this paper was published by Springer. This paper is for R & D purpose and Copyright [2011] Springer
 
Publisher Springer