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Antimicrobial Activity of Marine Sponge Clathria indica (Dendy, 1889)

DRS at CSIR-National Institute of Oceanography

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Title Antimicrobial Activity of Marine Sponge Clathria indica (Dendy, 1889)
 
Creator Ravichandran, S.
Wahidullah, S.
DeSouza, L.
Anbuchezhian, R.M.
 
Subject marine sponge
Clathria indica
antimicrobial peptide
Antimicrobial Activity
 
Description Sponges are sessile filter feeders that have developed efficient defense mechanisms against foreign invaders such as viruses, bacteria or eukaryotic organisms. Antimicrobial peptides are known as major components of the innate immune defense system in marine invertebrates. The aim of the present work was to study the antimicrobial properties of the Indian sponge Clathria indica with special reference to the identification of antimicrobial peptides. Crude methanolic extract and its chloroform, n-butanol and aqueous fractions were tested against 16 human pathogens which include eleven bacteria with four of them being multi-drug resistant and five pathogenic fungi. All fractions showed effective antibacterial activity against common and multi-drug resistant Salmonella typhi and antifungal activity against C. albicans and C. neoformans. However, they were ineffective against Escherichia coli, Pseudomonas aeruginosa, Streptococcus pyogenes and Staphylococcus aureus. Chloroform fraction being the most potent among the fractions tested on chemical investigation was indicative of the presence of peptides as evidenced by ninhydrin positive spots on TLC and presence of peptide bonds by NMR. Its ESI-MS showed presence of several peptides in the range of m/z 850 to 980. Structure of three peptides has been tentatively assigned by ESI-MS/MS or tandem mass analysis, on the basis of the amino acid sequence established. The results clearly show that the sponge C. indica represent an interesting source of marine invertebrates derived antimicrobial peptides in the development of new strategies to treat various infectious diseases.
 
Date 2011-08-23T09:35:50Z
2011-08-23T09:35:50Z
2011
 
Type Journal Article
 
Identifier Russian Journal of Bioorganic Chemistry, vol.37(4); 2011; 428-435
http://drs.nio.org/drs/handle/2264/3900
 
Language en
 
Rights This a preprint of the Materials accepted for publication in "Russian Journal of Bioorganic Chemistry". ? Pleiades Publishing, Ltd., 2011. http://www.springerlink.com/index/H185214375N2VT28.pdf
 
Publisher Pleiades Publishing