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Bacterial utilization of size-fractionated dissolved organic matter

DRS at CSIR-National Institute of Oceanography

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Title Bacterial utilization of size-fractionated dissolved organic matter
 
Creator Khodse, V.B.
Bhosle, N.B.
 
Subject dissolved organic matter
polysaccharides
primary production
bacteria
 
Description Dissolved organic matter (DOM) is an important source of organic carbon for sustaining the growth of heterotrophic bacteria. The bacterial utilization of high-molecular-weight (HMW; greater than 30 kDa to 0.22 mu m) and low-molecular-weight (LMW; greater than10 to 30 kDa) fractions of DOM was investigated. DOM was collected from a station in Dona Paula Bay, on the west coast of India, and fractionated into HMW and LMW portions. Each size fraction was inoculated with a natural population of bacteria and incubated over a period of 15 d at room temperature (28 + or - 2 degrees C); during this period, sub-samples were removed and the following were measured: dissolved organic carbon (DOC), total dissolved carbohydrate (TDCHO), free monosaccharide (MCHO), dissolved uronic acid (DURA), delta13C, bacterial abundance (BA), and bacterial production (BP). The LMW fraction was isotopically heavier (delta13C = ?23.7 to ?21.7ppt) than the HMW fraction (delta13C = ?27.0 to ?26.2ppt), and the initial TDCHO content of these fractions was 25 and 16%, respectively. The initial DURA content was similar in the LMW DOM (7.4% DOC) and the HMW DOM (7.0% DOC). BA and BP were consistently higher in the LMW DOM than in the HMW DOM. In 15 d incubation, greater proportions of DOC (35%), TDCHO (76%) and DURA (36%) were utilized in the LMW DOM than in the HMW DOM. This suggests that the LMW DOM was more biologically reactive, i.e. it was utilized more rapidly, than the HMW DOM. It appears that the bioreactivity and origin of the DOM strongly influenced its utilization by natural heterotrophic bacteria.
 
Date 2011-10-10T05:29:44Z
2011-10-10T05:29:44Z
2011
 
Type Journal Article
 
Identifier Aquatic Microbial Ecology, vol.64; 2011; 299-309
no
http://drs.nio.org/drs/handle/2264/3931
 
Language en
 
Relation Aquat_Microb_Ecol_64_299.jpg
 
Rights An edited version of this paper was published by Inter-Research. ? Inter-Research 2011
 
Publisher Inter-Research