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A novel halotolerant xylanase from marine isolate Bacillus subtilis cho40: gene cloning and sequencing

DRS at CSIR-National Institute of Oceanography

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Title A novel halotolerant xylanase from marine isolate Bacillus subtilis cho40: gene cloning and sequencing
 
Creator Khandeparker, R.
Verma, P.
Deobagkar, D.
 
Subject enzymes
biotechnology
Bacillus subtilis
seaweeds
 
Description A novel halotolerant xylanase from marine bacterium Bacillus subtilis cho40 isolated from Chorao island of Mandovi estuary Goa, India has been reported. Extracellular xylanase was produced by using agricultural residue such as wheat bran as carbon source under solid-state fermentation (SSF). The optimal pH and temperature of xylanase were reported to be 6.0 and 60 degrees C, respectively. Xyn40 was highly salt-tolerant, and showed highest activity at 0.5 M NaCl. Xylanase activity was greatly induced (140%) when pre-incubated with 0.5 M NaCl for 4 h. The xylanase gene, xyn40, from marine bacterium B. subtilis cho40 was cloned, and expressed in Escherichia coli. The xylanase gene was 645 bp long and had a 215 amino acid ORF protein with a molecular mass of 22.9 kDa. It had all features of xylanase enzyme and showed homology to xylanases reported from B. subtilis. It differs from the earlier reported xylanase sequences by the presence of more serine residues compared to threonine and also by the presence of polar (hydrophilic) amino acids in higher abundance (61%) than non-polar amino acids (39%). The novel xylanase, reported in this study is a halotolerant enzyme from marine isolate and can play a very important role in bioethanol production from marine seaweeds.
 
Date 2011-11-14T10:50:43Z
2011-11-14T10:50:43Z
2011
 
Type Journal Article
 
Identifier New Biotechnology, vol.28(6); 2011; 814-821
http://drs.nio.org/drs/handle/2264/3954
 
Language en
 
Rights An edited version of this paper was published by Elsevier. Copyright [2011] Elsevier
 
Publisher Elsevier