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Reducing Vibrio load in Artemia nauplii using antimicrobial photodynamic therapy: a promising strategy to reduce antibiotic application in shrimp larviculture

DRS at CSIR-National Institute of Oceanography

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Title Reducing Vibrio load in Artemia nauplii using antimicrobial photodynamic therapy: a promising strategy to reduce antibiotic application in shrimp larviculture
 
Creator Aparna, A.
Arshad, E.
Jasmin, C.
Pai, S.S.
BrightSingh, I.S.
Mohandas, A.
Anas, A.
 
Subject shrimp culture
bacterial diseases
product development
viral diseases
 
Description Antimicrobial photodynamic therapy (aPDT) as an alternative strategy to reduce the use of antibiotics in shrimp larviculture systems is proposed. The growth of a multiple antibiotic resistant Vibrio harveyi strain was effectively controlled by treating the cells with Rose Bengal and photosensitizing for 30 min using a halogen lamp. This resulted in the death of more than 50% of the cells within the first 10 min of exposure and the 50% reduction in the cell wall integrity after 30 min could be attributed to the destruction of outer membrane protein of V. harveyi by reactive oxygen intermediates produced during the photosensitization. Further, mesocosm experiments with V. harveyi and Artemia nauplii demonstrated that in 30 min, the aPDT could kill 78.9% and 91.2% of heterotrophic bacterial and Vibrio population respectively. In conclusion, the study demonstrated that aPDT with its rapid action and as yet unreported resistance development possibilities could be a propitious strategy to reduce the use of antibiotics in shrimp larviculture systems and thereby, avoid their hazardous effects on human health and the ecosystem at large.
 
Date 2012-01-17T09:42:36Z
2012-01-17T09:42:36Z
2012
 
Type Journal Article
 
Identifier Microbial Biotechnology , vol.5(1); 2012; 59-68
http://drs.nio.org/drs/handle/2264/4003
 
Language en
 
Rights ? 2011 The Authors
Microbial Biotechnology ? 2011 Society for Applied Microbiology and Blackwell Publishing Ltd
 
Publisher Society for Applied Microbiology