Echinicola shivajiensis sp. nov., a novel bacterium of the family "Cyclobacteriaceae" isolated from brackish water pond
DRS at CSIR-National Institute of Oceanography
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Title |
Echinicola shivajiensis sp. nov., a novel bacterium of the family "Cyclobacteriaceae" isolated from brackish water pond
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Creator |
Srinivas, T.N.R.
Tryambak, B.K. AnilKumar, P. |
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Subject |
bacteria
genetic isolation molecular structure Echinicola shivajiensis |
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Description |
Strain AK12 sup(T), an orange pigmented Gramnegative, rod shaped, non-motile bacterium, was isolated fromamud sample collected froma brackishwater pond at Rampur of West Bengal, India. The strain was positive for oxidase, catalase and phosphatase. The predominant fatty acids were iso-C sub(15:0) (42.7%), iso- C sub(17:0) 3OH (13.2%), C sub(16:1 omega 7c)/C sub(16:1 omega 6c) (summed feature 3) (8.0%), iso-C sub(17:1) I/anteiso-C sub(17:1) B (summed feature 4) (6.1%) and iso-C sub(17:1 omega 9c)/C sub(16:0) 10-methyl (summed feature 9) (9.4%). Strain AK12 sup(T) contained MK-7 as the major respiratory quinone and phosphatidylethanolamine, one unidentified aminophospholipid and six unidentified lipids as the polar lipids. The G+C content of DNA of the strain AK12 sup(T) was 46.2 mol%. The 16S rRNA gene sequence analysis indicated that strain AK12 sup(T) was member of the genus Echinicola and closely related to Echinicola vietnamensis, Echinicola pacifica and Echinicola jeungdonensis with pair-wise sequence similarity of 96.8, 96.3 and 96.0% respectively. Phylogenetic analyses indicated that the strain AK12 sup(T) clusteredwith E. vietnamensis and togetherwith E. pacifica and E. jeungdonensis with a phylogenetic distance of 5.1, 6.3 and 6.6% (94.9, 93.7 and 93.4% similarity) respectively. Based on data from the current polyphasic study, strain AK12 sup(T) is proposed as a novel species of the genus Echinicola, for which the name Echinicola shivajiensis sp. nov. is proposed. The type strain of E. shivajiensis is AK12 sup(T) (= MTCC 11083 sup(T) = JCM 17847 sup(T)).
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Date |
2012-02-27T06:39:16Z
2012-02-27T06:39:16Z 2012 |
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Type |
Journal Article
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Identifier |
Antonie van Leeuwenhoek, vol.101; 2012; 641-647
http://drs.nio.org/drs/handle/2264/4025 |
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Language |
en
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Rights |
An edited version of this paper was published by Springer. This paper is for R & D purpose and Copyright [2011] Springer.
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Publisher |
Springer
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