Biofilm-associated indole acetic acid producing bacteria and their impact in the proliferation of biofilm mats in solar salterns
DRS at CSIR-National Institute of Oceanography
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Title |
Biofilm-associated indole acetic acid producing bacteria and their impact in the proliferation of biofilm mats in solar salterns
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Creator |
Kerkar, S.
Raiker, L. Tiwari, A. Mayilraj, S. Dastager, S. |
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Subject |
bacteria
biofilm |
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Description |
Biofilm mats appear in salterns distinctively during the monsoon season when the salinity decreases below 12 percentile salinity units and within a short period cover the entire surface area of the saltern. A study was carried out in two salterns viz. Nerul and Curca to find a possible reason for the rapid proliferation of these solar biofilms. Out of the 125 bacteria isolated from these biofilms, 16 produced indole-3-acetic acid (IAA). Rapid in-situ assay with Salkowski reagent and HPLC analysis confirmed the IAA production. Four isolates consistently produced high IAA concentrations ranging from 9.5 to 14.2 mu g/mL in the presence of 4 mg/mL tryptophan concentrations in the growth media. The IAA-producing bacteria were Aeromonas aquariorum (N2), Pseudomonas alcaliphila (N3), Vibrio diazotrophicus (N6) and Pseudomonas pachastrellae (C3). These four IAA-producing bacteria also produced other growth promoting factors like ammonia. Three isolates produced siderophores and were phosphate solubilizers. There was enhancement in the growth of Cicer arietinum (length of the shoot and root) under axenic conditions and of biofilm mats (r = 0.9, p < 0.001; r = 0.8, p < 0.05 and r = 0.946, p < 0.01, respectively). This is, according to our knowledge, the first report indicating IAA-producing bacteria isolated from biofilms enhancing the proliferation of these biofilm mats in the solar salterns.
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Date |
2012-05-09T10:45:00Z
2012-05-09T10:45:00Z 2012 |
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Type |
Journal Article
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Identifier |
Biologia, vol.67(3); 2012; 454-460
http://drs.nio.org/drs/handle/2264/4051 |
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Language |
en
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Rights |
An edited version of this paper was published by Springer. This paper is for R & D purpose and Copyright [2011] Institute of Molecular Biology, Slovak Academy of Sciences.
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Publisher |
Institute of Molecular Biology, Slovak Academy of Sciences
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