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Characterization of Bacteria in Ballast Water Using MALDI-TOF Mass Spectrometry

DRS at CSIR-National Institute of Oceanography

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Title Characterization of Bacteria in Ballast Water Using MALDI-TOF Mass Spectrometry
 
Creator Emami, K.
Askari, V.
Ullrich, M.
Mohinudeen, K.
Anil, A.C.
Khandeparker, L.
Burgess, J.G.
Mesbahi, E.
 
Subject bacteria
ballast
mass spectroscopy
Vibrio
 
Description To evaluate a rapid and cost-effective method for monitoring bacteria in ballast water, several marine bacterial isolates were characterized by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). Since International Maritime Organization (IMO) regulations are concerned with the unintended transportation of pathogenic bacteria through ballast water, emphasis was placed on detecting species of Vibrio, enterococci and coliforms. Seawater samples collected from the North Sea were incubated in steel ballast tanks and the presence of potentially harmful species of Pseudomonas was also investigated. At the genus-level, the identification of thirty six isolates using MALDI-TOF MS produced similar results to those obtained by 16S rRNA gene sequencing. No pathogenic species were detected either by 16S rRNA gene analysis or by MALDI-TOF MS except for the opportunistically pathogenic bacterium Pseudomonas aeruginosa. In addition, in house software that calculated the correlation coefficient values (CCV) of the mass spectral raw data and their variation was developed and used to allow the rapid and efficient identification of marine bacteria in ballast water for the first time.
 
Date 2012-07-03T07:14:34Z
2012-07-03T07:14:34Z
2012
 
Type Journal Article
 
Identifier PLoS One, vol.7(6); 2012; e38515, 14pp.
http://drs.nio.org/drs/handle/2264/4077
 
Language en
 
Rights Copyright: 2012 Emami et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits
unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
 
Publisher Public Library of Science