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Differentially expressed genes under simulated deep-sea conditions in the psychrotolerant yeast Cryptococcus sp. NIOCCPY13

DRS at CSIR-National Institute of Oceanography

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Title Differentially expressed genes under simulated deep-sea conditions in the psychrotolerant yeast Cryptococcus sp. NIOCCPY13
 
Creator Singh, P.
Raghukumar, C.
Verma, A.K.
Meena, R.M.
 
Subject microorganisms
pressure tolerance
temperature tolerance
Cryptococcus sp.
 
Description Microorganisms exhibit varying degrees of tolerance to extreme conditions of pressure and temperature. In the present study, the psychrotolerant deep-sea yeast, Cryptococcus sp. NIOCC_PY13, was exposed to elevated hydrostatic pressure and low temperature to explore the differentially expressed genes responsible for its survival at such extreme conditions. The suppression subtractive hybridization technique was employed for identification of expressed upregulated genes at extreme conditions of pressure and temperature. The effect of elevated pressure was found to be different than that of combined pressure and temperature exposures. Altogether, 17 and 20 upregulated genes were identified at 50 MPa and 50 MPa/5 degrees C, respectively. These differentially expressed genes were similar to the NCBI database ESTs (expressed sequence tags), coding for proteins such as arachidonic acid metabolism, amino acid transport and unsaturation of membrane fatty acids, which have been previously demonstrated to assist in the survival of microorganisms under stress conditions. Interestingly, about 50 percent of the upregulated genes matched with hypothetical proteins at a percentage similarity of less than or equal to 96, suggesting their probability of being novel. Detailed studies of the above genes/proteins from deep-sea microorganisms are suggested for future investigations, which may shed more light on the existence and adaptation mechanisms adopted by these for their survival under such extreme conditions.
 
Date 2012-09-12T12:25:04Z
2012-09-12T12:25:04Z
2012
 
Type Journal Article
 
Identifier Extremophiles, vol.16; 2012; 777-785
no
http://drs.nio.org/drs/handle/2264/4163
 
Language en
 
Relation Extremophiles_16_777.jpg
 
Rights An edited version of this paper was published by Springer. This paper is for R & D purpose and Copyright [2012] Springer.
 
Publisher Springer