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Isolation and characterization of antibacterial compound from a mangrove-endophytic fungus, Penicillium chrysogenum MTCC 5108

DRS at CSIR-National Institute of Oceanography

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Title Isolation and characterization of antibacterial compound from a mangrove-endophytic fungus, Penicillium chrysogenum MTCC 5108
 
Creator PrabhaDevi
Rodrigues, C.
Naik, C.G.
DeSouza, L.
 
Subject fungi
antibiotics
genetic isolation
pharmacology
Penicillium chrysogenum
Porteresia coarctata
 
Description Microorganisms, especially endophytic fungi that reside in the tissue of living mangrove plants, seem to play a major role in meeting the general demand for new biologically active substances. During the course of screening for biologically active secondary metabolites from marine microorganisms, an antibiotic compound containing an indole and a diketopiperazine moiety was isolated from the culture medium of Penicillium chrysogenum, (MTCC 5108), an endophytic fungus on the mangrove plant Porteresia coarctata (Roxb.). The cell free culture medium of P. chrysogenum showed significant activity against Vibrio cholerae, (MCM B-322), a pathogen causing cholera in humans. Bioassay guided chemical characterization of the crude extract led to the isolation of a secondary metabolite possessing a molecular formula C sub(19)H sub(21)O sub(2)N sub(3). Its antibacterial activity was comparable with standard antibiotic, streptomycin. This compound (1) was found to be (3,1'-didehydro-3[2"(3'",3'"-dimethyl-prop-2-enyl)-3"-indolylmethylene]-6-methyl pipera-zine-2,5-dione) on the basis of mass spectrometry, infrared spectroscopy and one and two-dimensional nuclear magnetic resonance analysis.
 
Date 2013-01-09T11:21:05Z
2013-01-09T11:21:05Z
2012
 
Type Journal Article
 
Identifier Indian Journal of Microbiology, vol.52(4); 2012; 617-623
http://drs.nio.org/drs/handle/2264/4224
 
Language en
 
Rights An edited version of this paper was published by Springer. This paper is for R & D purpose and ? Association of Microbiologists of India 2012
 
Publisher Association of Microbiologists of India