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Modulation of LPS induced inflammatory response by Lawsonyl monocyclic terpene from the marine derived Streptomyces sp.

DRS at CSIR-National Institute of Oceanography

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Title Modulation of LPS induced inflammatory response by Lawsonyl monocyclic terpene from the marine derived Streptomyces sp.
 
Creator Ali, A.
Khajuria, A.
Sidiq, T.
AshokKumar
Thakur, N.L.
Naik, D.
Vishwakarma, R.A.
 
Subject pharmacology
phytohormones
macrophages
Streptomyces sp.
 
Description In continuing research for compounds with immunosuppressive activity, Lawsonone (1), a novel Lawsonyl derivative isolated from marine-derived bacteria Streptomyces sp. was evaluated for its potent immunosuppressive activity on immune system. The effect of Lawsonone (1) was elucidated on the immune cells (splenocytes and macrophages) collected from BALB/c mice. Study was carried out to find the effect of Lawsonone (1) on Con-A and LPS stimulated splenocyte proliferation, LPS-induced NO, IL-1beta, IL-6 and TNF-alpha production in macrophages. Furthermore, the effect of Lawsonone (1) on T-cell subsets (CD4 and CD8) and total B-cell (CD19) population was analyzed by flow cytometry. The results obtained in the present study showed that Lawsonone (1) inhibited the proliferation of both T and B splenocytes. It inhibited the nitric oxide (NO) and pro-inflammatory cytokine (IL-1beta, IL-6 and TNF-alpha) production in LPS-stimulated macrophages in a dose-dependent manner. Moreover, flow cytometric analysis indicated the prominent inhibition of CD4, CD8 and CD19 cell populations in the spleen of mice treated with the variable doses of Lawsonone (1), with the maximum inhibition at the lowest dose (0.1 mu M). Taken together, the present results suggest that Lawsonone (1) may act as a potent molecule for immunosuppression and anti-inflammation, supporting its immunopharmacologic application to modify the immune system.
 
Date 2013-04-01T09:54:49Z
2013-04-01T09:54:49Z
2013
 
Type Journal Article
 
Identifier Immunology Letters, vol.150; 2013; 79-86
http://drs.nio.org/drs/handle/2264/4265
 
Language en
 
Rights An edited version of this paper was published by Elsevier. Copyright [2013] Elsevier
 
Publisher Elsevier