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DNA barcodes for marine fungal identification and discovery

DRS at CSIR-National Institute of Oceanography

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Title DNA barcodes for marine fungal identification and discovery
 
Creator Velmurugan, S.
Prasannakumar, C.
Manokaran, S.
AjithKumar, T.
Samkamaleson, A.
Palavesam, A.
 
Subject Fungi
Species
Identification
Phylogenetics
 
Description We employed DNA barcodes for identification of fungal species in marine sediments. Sediments were collected seasonally along the Southeast coast of India from which a culturable fungal library was constructed. All cultured species were morphologically documented using microscopical analysis. A maximum population density of 19.3 ? 103 CFU/g was recorded in monsoon and minimum of 3 ? 103 CFU/g in premonsoon season. Two-way analysis of variance suggests that the fungal community varied significantly between the seasons (F = 9.543, P < 0.001) and at various depths sampled (F = 4.655, P < 0.05). In total, 54 fungal species belonging to 13 different families were documented and all species were sequenced for internal transcribed spacer genes. Each species was represented by at least two specimens constituting a total of 171 specimens for DNA barcoding. Twelve species of a marine fungi were sequenced for the first time. Branching patterns of phylogenetic tree strongly supported the sequence variations within and between all species barcoded. Based on the pairwise distance model we suggest barcode gaps of 15 %, 21 %, 30 %, 35 % and 51 % for genera, family, order, class and phyla respectively.
 
Date 2014-01-20T06:18:28Z
2014-01-20T06:18:28Z
2013
 
Type Journal Article
 
Identifier Fungal Ecology, vol.6(5); 2013; 408-418
http://drs.nio.org/drs/handle/2264/4444
 
Language en
 
Rights An edited version of this paper was published by Elsevier. Copyright [2013] Elsevier
 
Publisher Elsevier