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Mobilization of manganese by basalt associated Mn(II)-oxidizing bacteria from the Indian Ridge System

DRS at CSIR-National Institute of Oceanography

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Title Mobilization of manganese by basalt associated Mn(II)-oxidizing bacteria from the Indian Ridge System
 
Creator Sujith, P.P.
Mourya, B.S.
Krishnamurthi, S.
Meena, R.M.
LokaBharathi, P.A.
 
Subject redox reactions
bacteria
basalts
manganese
ridges
organic carbon
 
Description The Indian Ridge System basalt bearing Mn-oxide coatings had todorokite as the major and birnesite as the minor mineral. We posit that microorganisms associated with these basalts participate in the oxidation of Mn and contribute to mineral deposition. We also hypothesized that, the Mn-oxidizing microbes may respond reversibly to pulses of fresh organic carbon introduced into the water column by mobilizing the Mn in Mn-oxides. To test these two hypotheses, we enumerated the number of Mn-oxidizers and -reducers and carried out studies on the mobilization of Mn by microbial communities associated with basalt. In medium containing 100 mew M Mn2+, 103 colony forming units (CFU) were recovered with undetectable number of reducers on Mn-oxide amended medium, suggesting that the community was more oxidative. Experiments were then conducted with basalt fragments at 4 ± 2 °C in the presence ‘G+’ and absence ‘G-’ of glucose (0.1%). Controls included set-ups, some of which were poisoned with 15 mM azide and the others of which were heat-killed. The mobilization of Mn in the presence of glucose was 1.76 mew g g-1 d-1 and in the absence, it was 0.17 mew g g-1 d-1 after 150 d. Mn mobilization with and without added glucose was 13 and 4 times greater than the corresponding azide treated controls. However, rates in ‘G+’ were 16 times and ‘G-’ 24 times more than the respective heat killed controls. The corresponding total counts in the presence of added glucose increased from 1.63 × 106 to 6.71 × 107 cells and from 1.41 × 107 to 3.52 × 107 cells g-1 in its absence. Thus, the addition of glucose as a proxy for organic carbon changed the community’s response from Mn(II)-oxidizing to Mn(IV)-reducing activity. The results confirm the participation of Mn oxidizing bacteria in the mobilization of Mn. Identification of culturable bacteria by 16S rRNA gene analysis showed taxonomic affiliations to Bacillus, Exiguobacterium, Staphylococcus, Brevibacterium and Alcanivorax sp.
 
Date 2014-02-05T09:20:34Z
2014-02-05T09:20:34Z
2014
 
Type Journal Article
 
Identifier Chemosphere, vol.95; 2014; 486-495.
no
http://drs.nio.org/drs/handle/2264/4449
 
Language en
 
Relation Bioorg_Med_Chem_Lett_24_2863.jpg
 
Rights An edited version of this paper was published by Elsevier. Copyright [2013] Elsevier
 
Publisher Elsevier