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Full-length cloning and phylogenetic analyses of translationally controlled tumour protein and ferritin genes from the Indian white prawn, Fenneropenaeus indicus (H. Milne Edwards)

DRS at CSIR-National Institute of Oceanography

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Title Full-length cloning and phylogenetic analyses of translationally controlled tumour protein and ferritin genes from the Indian white prawn, Fenneropenaeus indicus (H. Milne Edwards)
 
Creator Nayak, S.
Ramaiah, N.
Meena, R.M.
Sreepada, R.A.
 
Subject phylogenetic analyses
protein
gene sequence
disease
 
Description Elucidation, through molecular analyses, of bacterial afflictions in commercially important aquaculture- reared shrimps is pivotal for the prevention and/or control of disease outbreaks. In this study, we examined the phylogenetic relatedness and compared the possible immune-related functional roles of both translationally controlled tumour protein (TCTP) and ferritin genes with previous studies. Both TCTP and ferritin genes were substantially upregulated in the Indian white prawn, Fenneropenaeus indicus (H. Milne Edwards), post-larvae following bath challenge with the virulent strain of bacteria, Vibrio harveyi D3. Full-length cloning of these genes by rapid amplification of complementary DNA ends -polymerase chain reaction (RACEPCR) yielded 727-base pair (bp)-long TCTP and 1212-bp-long ferritin gene sequences. Their open reading frames (ORFs) were 507 and 510 bp, respectively. The TCTP-ORF coded for 168 amino acids with three substitutions at positions 37, 141, 155, and the ferritin ORF coded for 170 amino acids with no species-specific substitutions. Phylogenetic analysis suggested the closest relatedness of both TCTP and ferritin from F. indicus to Chinese white prawn, Fenneropenaeus chinensis (Osbeck). In addition to reporting the full-length sequences of these immune-relevant genes, this study highlighted their conserved natures, which perhaps make them important defence-related proteins in the innate immune system of F. indicus.
 
Date 2014-02-05T09:35:05Z
2014-02-05T09:35:05Z
2014
 
Type Journal Article
 
Identifier Journal of Fish Diseases, vol.37; 2014; 77?87.
http://drs.nio.org/drs/handle/2264/4453
 
Language en
 
Rights An edited version of this paper was published by John Wiley & Sons. Copyright ? 2013 John Wiley & Sons, Inc. All Rights Reserved.
 
Publisher John Wiley