Record Details

Manipulating galectin expression in zebrafish (Danio rerio)

DRS at CSIR-National Institute of Oceanography

View Archive Info
 
 
Field Value
 
Title Manipulating galectin expression in zebrafish (Danio rerio)
 
Creator Feng, C.
Nita-Lazar, M.
Gonzalez-Montalban, N.
Wang, J.
Mancini, J.
Ravindran, C.
Ahmed, H.
Vasta, G.R.
 
Subject gene expression
public health
diseases
Danio rerio
 
Description Techniques for disrupting gene expression are invaluable tools for the analysis of the biological role(s) of a gene product. Because of its genetic tractability and multiple advantages over conventional mammalian models, the zebrafish (Danio rerio) is recognized as a powerful system for gaining new insight into diverse aspects of human health and disease. Among the multiple mammalian gene families for which the zebrafish has shown promise as an invaluable model for functional studies, the galectins have attracted great interest due to their participation in early development, regulation of immune homeostasis, and recognition of microbial pathogens. Galectins are Beta-galactosyl-binding lectins with a characteristic sequence motif in their carbohydrate recognition domains (CRDs), which comprise an evolutionary conserved family ubiquitous in eukaryotic taxa. Galectins are emerging as key players in the modulation of many important pathological processes, which include acute and chronic inflammatory diseases, autoimmunity and cancer, thus making them potential molecular targets for innovative drug discovery. Here, we provide a review of the current methods available for the manipulation of gene expression in the zebrafish, with a focus on gene knockdown [morpholino (MO)-derived antisense oligonucleotides] and knockout (CRISPR-Cas) technologies.
 
Date 2015-02-11T04:22:39Z
2015-02-11T04:22:39Z
2015
 
Type Book Chapter
 
Identifier Galectins:Methods and protocols. ed. by: Stowell, S.R.; Cummings, R.D.. Springer; New York; USA; 2015; 327-341
http://drs.nio.org/drs/handle/2264/4679
 
Language en
 
Relation Methods in molecular biology: Methods and protocols; 1207; Walker, J.M.; ed.
 
Rights Copyright [2015] Springer
 
Publisher Springer