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Effect of biofilm on fluorescence measurements derived from fast repetition rate fluorometers

DRS at CSIR-National Institute of Oceanography

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Title Effect of biofilm on fluorescence measurements derived from fast repetition rate fluorometers
 
Creator Patil, J.S.
Saino, T.
 
Subject CHEMISTRY AND BIOGEOCHEMISTRY
 
Description This study evaluates, for the first time, the influence of biofilms on single and double optical window (SOW and DOW, respectively) fast repetition rate fluorometer (FRRF) measurements of microalgal photosystem-II initial fluorescence (F0), maximum fluorescence (Fm), variable fluorescence (Fv= Fm – F0), quantum yield(Fv/Fm) and functional absorption cross section (σPSII)]. Biofilms with chlorophyll > 0.1 μg cm-2 and > 0.3 μgcm-2 on SOW and DOW, respectively, produced a substantial increase in fluorescence. However, the relative magnitude of biofouling effects depended on sample chlorophyll concentrations, being more critical at concentrations < 1 mg m-3. In DOW-FRRF, biofilms affected F0 (increased) and Fv/Fm) (decreased) but not Fv and σPSII, whereas in SOW-FRRF, biofilms increased fluorescence and showed a variable effect on Fv Fm and σPSII, because only biofilms on SOW attained actual Fm. As a result, the biofilm effect was substantial on SOW-FRRF measurements. On the other hand, the neutral-density filters (representing non-chlorophyll containing biofilms) with different transmission levels reduced the fluorescence signal. Correction procedures for the above photosystem-II parameters are proposed here.
 
Date 2015-10-01T13:13:12Z
2015-10-01T13:13:12Z
2015
 
Type Journal Article
 
Identifier Biofouling, vol.31(5); 2015; 417-432
no
http://drs.nio.org/drs/handle/2264/4793
 
Language en
 
Relation Biofouling_31_417.jpg
 
Rights The final and definitive form of the preprint has been published in the "Biofouling" © 2015 Taylor & Francis; "Biofouling" is available online at http://www.tandfonline.com with open URL of article : http://dx.doi.org/10.1080/08927014.2015.1052416. The term & condition associated with this preprint is at http://www.tandfonline.com/page/terms-and-conditions
 
Publisher Taylor and Francis