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Genotoxic potency of mercuric chloride in gill cells of marine gastropod Planaxis sulcatus using comet assay

DRS at CSIR-National Institute of Oceanography

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Title Genotoxic potency of mercuric chloride in gill cells of marine gastropod Planaxis sulcatus using comet assay
 
Creator Bhagat, J.
Ingole, B.S.
 
Subject BIOLOGY::Physiology, biochemistry, biophysics
AQUATIC POLLUTION::Effects on organisms
MOLLUSCS::Physiology, biochemistry, biophysics
 
Description In vivo and in vitro exposures were used to investigate the genotoxicity of mercuric chloride (HgCl2) to the marine snail, Planaxis sulcatus. The comet assay protocol was validated on gill cells exposed in vitro to hydrogen peroxide (H2O2, 0?50 ?M). Snails were exposed in vivo for 96 h to HgCl2 (10, 20, 50, and 100 ?g/l). Our results showed significant concentration-dependent increase in the tail DNA (TDNA) and olive tail moment (OTM) in exposed snails for all doses compared with controls. In vitro exposure to HgCl2(10?100 ?g/l) resulted in significantly higher values for TDNA at all concentrations. Our results showed that DNA damage increased in the gill cell with increasing exposure time. This study demonstrates the usefulness of comet assay for detection of DNA damage after exposure to HgCl2 and the sensitivity of marine snail P. sulcatus as a good candidate species for metal pollution
 
Date 2015-10-16T05:50:56Z
2015-10-16T05:50:56Z
2015
 
Type Journal Article
 
Identifier Environmental Science and Pollution Research, vol.22(14); 2015; 10758-10768
http://drs.nio.org/drs/handle/2264/4795
 
Language en
 
Rights An edited version of this paper was published by Springer. This paper is for R & D purpose and Copyright [2015] Springer.
 
Publisher Springer