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Unicellular cyanobacteria synechocystis accommodate heterotrophic bacteria with varied enzymatic and metal resistance properties

DRS at CSIR-National Institute of Oceanography

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Title Unicellular cyanobacteria synechocystis accommodate heterotrophic bacteria with varied enzymatic and metal resistance properties
 
Creator Anas, A.
Sageer, S.
Jasmin, C.
Vijayan, V.
Pavanan, P.
Athiyanathil, S.
Nair, S.
 
Subject Microbiology::General
Aquatic ecology, productivity::Biological productivity
Aquatic ecology, productivity::Ecosystems and energetics
Aquatic ecology, productivity::Species interactions
 
Description The interactions between heterotrophic bacteria and primary producers have a profound impact on the functioning of marine ecosystem. We characterized the enzymatic and metal resistance properties of fourteen heterotrophic bacteria isolated from a unicellular cyanobacterium Synechocystis sp. that came from a heavy metal contaminated region of Cochin estuary, southwest coast of India. Based on 16S rRNA gene sequence similarities, the heterotrophic bacteria were grouped into three phyla: namely Actinobacteria, Firmicute, and Proteobacteria. Overall Proteobacteria showed a higher level of enzyme expression while Actinobacteria and Firmicutes showed higher tolerance to heavy metals. Among Proteobacteria, an isolate of Marinobacter hydrocarbonoclasticus (MMRF-584) showed highest activities of Beta-glucosidase (1.58?0.2micro Mml-1 min-1) and laminarinase (1170.17?95.4micro gml-1 min-1), while other two isolates of M. hydrocarbonoclasticus, MMRF-578 and 581, showed highest phosphatase (44.71?0.2micro Mml-1 min-1) and aminopeptidase (33.22?0 micro Mml-1min-1) activities respectively. Among Firmicutes, the Virgibacillus sp. MMRF-571 showed exceptional resistance against the toxic heavy metals Cd (180 mM), Pb (150 mM), and Hg (0.5 mM). Bacillus cereus, MMRF-575, showed resistance to the highest concentrations of Co (250 mM), Cd (150 mM), Pb (180 mM), Hg (0.5 mM), Ni (280 mM), and Zn (250 mM) tested. Our results show that heterotrophic bacteria with varied enzymatic and metal resistance properties are associated with Synechocystis sp. Further studies to delineate the role of these heterotrophic bacteria in protecting primary producers from toxic effects of heavy metals and their potential application in bioremediation will be appreciated
 
Date 2016-10-13T12:24:41Z
2016-10-13T12:24:41Z
2016
 
Type Journal Article
 
Identifier Journal of Basic Microbiology, vol.56(8); 2016; 845-856
http://drs.nio.org/drs/handle/2264/5019
 
Language en
 
Rights This is a preprint of an article accepted for publication in Journal of Basic Microbiology. Copyright ? [2016] WILEY-VCH Verlag GmbH & Co. ?. After publication of the Contribution by Wiley, the preprint notice should be amended to read as follows: ?This is a preprint of an article published in [Journal of Basic Microbiology, vol.56(8); 2016; 845-856]?and an electronic link to the Journal?s WWW site, located at the following Wiley URL: http://onlinelibrary.wiley.com/doi/10.1002/jobm.201500693/full. The Contributor agrees not to update the preprint or replace it with the published version of the Contribution.
 
Publisher WILEY-VCH Verlag GmbH & Co.