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Moult-inhibiting fusion protein augments while polyclonal antisera attenuate moult stages and duration in Penaeus monodon

DRS at CSIR-National Institute of Oceanography

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Title Moult-inhibiting fusion protein augments while polyclonal antisera attenuate moult stages and duration in Penaeus monodon
 
Creator Vrinda, S.
Jasmin, C.
Sivakumar, K.C.
Jose, B.
Philip, R.
BrightSingh, I.S.
 
Subject Crustaceans::Reproduction and development
Crustaceans::Physiology, biochemistry, biophysics
Crustaceans::General
 
Description Moulting in crustaceans is regulated by moult-inhibiting hormone (MIH) of the CHH family neuropeptides. The inhibitory functions of MIH have pivotal roles in growth and reproduction of Penaeus monodon. In this study, we report the expression of a thioredoxin-fused mature MIH I protein (mf-PmMIH I) of P. monodon in a bacterial system and its use as antigen to raise polyclonal antiserum (anti-mf-PmMIH I). The mature MIH I gene of 231 bp, that codes for 77 amino acids, was cloned into the Escherichia coli thioredoxin gene fusion expression system. The translation expression vector construct (mf-PmMIH I+pET32a+) upon induction produced 29.85 kDa mature MIH I fusion protein (mf-PmMIH I). The purified fusion protein was used as exogenous MIH I and as antigen to raise polyclonal antisera. When fusion protein (mf-PmMIH I) was injected into D2 and D3 stages of juvenile shrimp, the moult cycle duration was extended significantly to 16.67 ? 1.03 and 14.67 ? 1.03 days respectively compared to that of 11.67 ? 1.03 days in controls. Moult duration was further reduced to 8.33 ? 0.82 days when polyclonal antiserum (anti-mf-PmMIH I ? 1:500 dilutions) was injected. Anti-mf-PmMIH I immunolocalized MIH I producing neurosecretory cells in the eyestalk of P. monodon. In short, the present manuscript reports an innovative means of moult regulation in P. monodon with thioredoxin fused MIH I and antisera developed
 
Date 2016-10-17T07:42:59Z
2016-10-17T07:42:59Z
2016
 
Type Journal Article
 
Identifier General and Comparative Endocrinolog, vol.233(1); 2016; 32-42
http://drs.nio.org/drs/handle/2264/5038
 
Language en
 
Rights Copyright [2016]. All efforts have been made to respect the copyright to the best of our knowledge. Inadvertent omissions, if brought to our notice, stand for correction and withdrawal of document from this repository
 
Publisher Elsevier