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Regulating gonad inhibition and vitellogenin/vitellin induction in Penaeus monodon using mature GIH fusion protein and polyclonal antisera

DRS at CSIR-National Institute of Oceanography

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Title Regulating gonad inhibition and vitellogenin/vitellin induction in Penaeus monodon using mature GIH fusion protein and polyclonal antisera
 
Creator Vrinda, S.
Jasmin, C.
Sivakumar, K.C.
Jose, S.
Jose, B.
Philip, R.
BrightSingh, I.S.
 
Subject Crustaceans::Physiology, biochemistry, biophysics
Crustaceans::Reproduction and development
 
Description Gonad inhibiting hormone (GIH), type II class of the CHH family neuropeptides, is released by the neurohaemal XO-SG complex of the eyestalk. The inhibitory function of GIH has a pivotal role in gonad development and reproduction. In this study, we report the expression and production of a thioredoxin-fused mature GIH protein (mf-PmGIH) of Penaeus monodon in a bacterial system and its use as antigen to raise polyclonal antiserum (anti-mf-PmGIH). The mature GIH gene of 237 bp that codes for 79 amino acids, was cloned into the Escherichia coli thioredoxin gene fusion expression system. The expression vector construct (mf-PmGIH + pEt32a +) upon induction produced 32.16 kDa mature GIH fusion protein (mf-PmGIH)?The purified fusion protein was used as exogenous GIH and as antigen to raise polyclonal antisera. The fusion protein when injected into juvenile shrimp significantly reduced vitellogenin/vitellin levels by 31.55% within 72 h in comparison to the controls showing the gonad inhibiting property. Vitellogenin/vitellin levels were significantly induced by 74.10% within 6 h when polyclonal antiserum (anti-mf-PmGIH-1:500) was injected in P.monodon. Anti-mf-PmGIH immunolocalized GIH producing neurosecretory cells in the eyestalk of P.monodon. The present manuscript reports an innovative means of gonad inhibition and vitellogenin/vitellin induction with thioredoxin fused GIH and antisera developed
 
Date 2017-03-01T08:53:19Z
2017-03-01T08:53:19Z
2017
 
Type Journal Article
 
Identifier Comparative Biochemistry and Physiology, Part A, vol.203; 2017; 167-178
http://drs.nio.org/drs/handle/2264/5076
 
Language en
 
Rights An edited version of this paper was published by Elsevier. Copyright [2016] Elsevier
 
Publisher Elsevier