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Biotransformation and detoxification of xylidine orange dye using immobilized cells of marine-derived Lysinibacillus sphaericus D3

DRS at CSIR-National Institute of Oceanography

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Title Biotransformation and detoxification of xylidine orange dye using immobilized cells of marine-derived Lysinibacillus sphaericus D3
 
Creator PrabhaDevi
Wahidullah, S.
Sheikh, F.
Pereira, R.
Narkhede, N.
Amonkar, D.
Tilvi, S.
Meena, R.M.
 
Subject Microbiology
Aquatic resources
Marine technology
 
Description Lysinibacillus sphaericus D3 cell-immobilized beads in natural gel sodium alginate decolorized the xylidine orange dye 1-(dimethylphenylazo)-2-naphthol-6-sulfonic acid sodium salt in the laboratory. Optimal conditions were selected for decolorization and the products formed were evaluated for toxicity by disc diffusion assay against common marine bacteria which revealed the non-toxic nature of the dye-degraded products. Decolorization of the brightly colored dye to colorless products was measured on an Ultra Violet-Vis spectrophotometer and its biodegradation products monitored on Thin Layer Chromatographic plate and High Performance Liquid Chromatography (HPLC). Finally, the metabolites formed in the decolorized medium were characterized by mass spectrometry. This analysis confirms the conversion of the parent molecule into lower molecular weight aromatic phenols and sulfonic acids as the final products of biotransformation. Based on the results, the probable degradation products of xylidine orange were naphthol, naphthylamine-6-sulfonic acid, 2-6-dihydroxynaphthalene, and bis-dinaphthylether. Thus, it may be concluded that the degradation pathway of the dye involved (a) reduction of its azo group by azoreductase enzyme (b) dimerization of the hydrazo compound followed by (c) degradation of monohydrazo as well as dimeric metabolites into low molecular weight aromatics. Finally, it may be worth exploring the possibility of commercially utilizing L. sphaericus D3 for industrial applications for treating large-scale dye waste water
 
Date 2017-03-01T11:12:11Z
2017-03-01T11:12:11Z
2017
 
Type Journal Article
 
Identifier Marine Drugs, vol.15(30); 2017; 1-14
http://drs.nio.org/drs/handle/2264/5100
 
Language en
 
Rights Copyright [2017]. All efforts have been made to respect the copyright to the best of our knowledge. Inadvertent omissions, if brought to our notice, stand for correction and withdrawal of document from this repository.
 
Publisher MPDI