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Gene expression atlas of pigeonpea and its application to gain insights into genes associated with pollen fertility implicated in seed formation

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Relation http://oar.icrisat.org/9953/
http://dx.doi.org/10.1093/jxb/erx010
10.1093/jxb/erx010
 
Title Gene expression atlas of pigeonpea and its application to gain insights into genes associated with pollen fertility implicated in seed formation
 
Creator Pazhamala, L T
Purohit, S
Saxena, R K
Garg, V
Krishnamurthy, L
Verdier, J
Varshney, R K
 
Subject Pigeonpea
Genetics and Genomics
 
Description Pigeonpea (Cajanus cajan) is an important grain legume of the semi-arid tropics, mainly used for its protein rich seeds. To link the genome sequence information with agronomic traits resulting from specific developmental processes, a Cajanus cajan gene expression atlas (CcGEA) was developed using the Asha genotype. Thirty tissues/organs representing developmental stages from germination to senescence were used to generate 590.84 million paired-end RNA-Seq data. The CcGEA revealed a compendium of 28 793 genes with differential, specific, spatio-temporal and constitutive expression during various stages of development in different tissues. As an example to demonstrate the application of the CcGEA, a network of 28 flower-related genes analysed for cis-regulatory elements and splicing variants has been identified. In addition, expression analysis of these candidate genes in male sterile and male fertile genotypes suggested their critical role in normal pollen development leading to seed formation. Gene network analysis also identified two regulatory genes, a pollen-specific SF3 and a sucrose-proton symporter, that could have implications for improvement of agronomic traits such as seed production and yield. In conclusion, the CcGEA provides a valuable resource for pigeonpea to identify candidate genes involved in specific developmental processes and to understand the well-orchestrated growth and developmental process in this resilient crop.
 
Publisher Oxford University Press on behalf of the Society for Experimental Biology
 
Date 2017-04-01
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://oar.icrisat.org/9953/1/erx010.pdf
Pazhamala, L T and Purohit, S and Saxena, R K and Garg, V and Krishnamurthy, L and Verdier, J and Varshney, R K (2017) Gene expression atlas of pigeonpea and its application to gain insights into genes associated with pollen fertility implicated in seed formation. Journal of Experimental Botany, 68 (8). pp. 2037-2054. ISSN 0022-0957