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Probiotic Potential Streptomyces Species From The Grains Of Pearl Millet (Pennisetum Glaucum)

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Relation http://oar.icrisat.org/9959/
http://doi.org/10.5897/AJMR2017.8469
10.5897/AJMR2017.8469
 
Title Probiotic Potential Streptomyces Species From The Grains Of Pearl Millet (Pennisetum Glaucum)
 
Creator Kunchala, R
Durgalla, P
Banerjee, R
Mazumdar, S D
Srinivas, V
Gopalakrishnan, S
 
Subject Pearl Millet
 
Description Actinomycetes have been demonstrated for plant growth-promotion, antagonistic against plant
pathogens and insect pests and biofortification traits in many agriculturally important crops. The
present investigation was conducted to characterize probiotic properties of actinomycete(s) isolated
from pearl millet flour and batter samples. A media selective and specific were used for isolation,
actinomycetes isolation agar (AIA), and the most prominent actinomycete (found abundantly in the AIA
plate) was isolated and maintained on AIA slants at 4°C for further investigation. The most prominent
actinomycete was characterized for traits including Gram staining, morphology (such as color, margin,
size, shape, elevation, form and surface), biochemistry (such as urease, catalase, oxidase, hydrogen
sulphide, nitrogen reduction, gelatin liquefaction, starch hydrolysis and carbohydrate utilization), IMViC
tests (such as indole, methyl red, Voges Proskauer and citrate utilization), probiotic potentials (such as
acid [pH 2, 3], bile [0.5%], NaCl [6 and 9%], phenol tolerance [0.4%]), antibiotic tolerance (such as
tetracycline, streptomycin, kanamycin, chloramphenicol, ciprofloxacin, ampicillin, penicillin,
erythromycin and vancomycin) and antimicrobial activities against human pathogens (such as
Escherichia coli, Staphylococcus aureus and Salmonella typhi). It was possible to isolate only one
probiotic actinomycete based on the properties. The sequences of 16s rDNA gene of the actinomycete
was matched with Streptomyces species in BLAST analysis. The sequences of the Streptomyces spp.
were submitted to NCBI and accession number obtained. This study indicated that the selected
Streptomyces spp. could be used to develop new probiotic foods.
 
Publisher Academic Journals
 
Date 2017-04
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://oar.icrisat.org/9959/1/Rajyalakshmi%20et%20al.pdf
Kunchala, R and Durgalla, P and Banerjee, R and Mazumdar, S D and Srinivas, V and Gopalakrishnan, S (2017) Probiotic Potential Streptomyces Species From The Grains Of Pearl Millet (Pennisetum Glaucum). African Journal of Microbiology Research, 11 (14). pp. 553-559. ISSN 1996-0808