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Evaluation of Various Tissues of the Caerulean Damsel, Pomacentrus caeruleus for Initiating In Vitro Cell Culture Systems

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Relation http://eprints.cmfri.org.in/11777/
https://link.springer.com/article/10.1007/s40011-016-0751-x
10.1007/s40011-016-0751-x
 
Title Evaluation of Various Tissues of the Caerulean Damsel,
Pomacentrus caeruleus for Initiating In Vitro Cell Culture
Systems
 
Creator George, Githa Ann
Sobhana, K S
Sheethal, Mary Sunny
Sreedevi, S
 
Subject Fish Biotechnology
 
Description Explantation and trypsinisation methods for tissue
dissociation were attempted for the establishment of
primary cell cultures from the caerulean damsel, Pomacentrus
caeruleus. Among the tissues taken, fin, liver and
caudal peduncle showed good attachment with emergence
of cells. The cells were best suited to grow in Leibovitz’s
L-15 basal medium supplemented with foetal bovine serum
(initially 20 % which was later reduced to 5–10 % during
subsequent passages) at an ambient temperature of
28 ± 2 �C and pH 7.2 ± 0.2. These cultures persisted at
temperatures from 17 to 32 �C, and proliferated at temperatures
from 24 to 30 �C. The cells have been cryopreserved
successfully with a survival rate of 80 %. Results
suggest that fin, caudal peduncle and liver cell cultures
have potential for development into cell lines.
 
Publisher National Academy of Sciences, India
 
Date 2016
 
Type Article
PeerReviewed
 
Format text
 
Language en
 
Identifier http://eprints.cmfri.org.in/11777/1/Evaluation%20of%20Various%20Tissues%20of%20the%20Caerulean%20Damsel_Proceeings%20of%20the%20National%20Academy%20of%20Sciences_K%20S%20Sobhana_2016.pdf
George, Githa Ann and Sobhana, K S and Sheethal, Mary Sunny and Sreedevi, S (2016) Evaluation of Various Tissues of the Caerulean Damsel, Pomacentrus caeruleus for Initiating In Vitro Cell Culture Systems. Proceedings of the National Academy of Sciences Section B Biological Sciences. pp. 1-11.