Record Details

Genetic variation and phylogenetic relationship between two species of yellow catfish, Horabagrus brachysoma and H. nigricollaris (Teleostei: Horabagridae) based on RAPD and microsatellite markers

CMFRI Repository

View Archive Info
 
 
Field Value
 
Relation http://eprints.cmfri.org.in/11787/
https://www.ncbi.nlm.nih.gov/pubmed/20862549
10.1007/s11033-010-0352-3
 
Title Genetic variation and phylogenetic relationship between
two species of yellow catfish, Horabagrus brachysoma
and H. nigricollaris (Teleostei: Horabagridae) based
on RAPD and microsatellite markers
 
Creator Abdul Muneer, P M
Gopalakrishnan, A
Shivanandan, Remya
Basheer, V S
Ponniah, A G
 
Subject Fish Genetics
 
Description The two species of yellow catfish, Horabagrus
brachysoma and H. nigricollaris are categorized as
‘endangered’ and ‘critically endangered’ respectively in
their wild habitat. Proper knowledge of genetic structure and
variability of these endangered species are highly essential
for the management, conservation and improvement of fish
stocks. Therefore, genetic variation and phylogenetic relationships
between these species of yellow catfish sampled
from Chalakkudy River in the hot spot of biodiversity-
Western Ghats region, Kerala, India were analyzed by using
Random amplified polymorphic DNA (RAPD) and microsatellite
markers. 85 RAPD and five microsatellites loci
were detected to analyze the genetic variation and phylogenetic
relationships among these species. Out of 85 RAPD
loci produced only 52.94% were polymorphic whereas in
microsatellite, all 5 loci were polymorphic (100%). Species specific
RAPD bands were found in both species studied. In
microsatellite, the number of alleles across the five loci
ranged from 1 to 8. The observed heterozygosities in
H. brachysoma and H. nigricollaris were 0.463 and 0.443,
respectively. Here, both RAPD and microsatellite methods
reported a low degree of gene diversity and lack of genetic
heterogeneity in both species of Horabagrus which strongly
emphasize the need of fishery management, conservation
and rehabilitation of these species.
 
Publisher Kluwer
 
Date 2011
 
Type Article
PeerReviewed
 
Format text
 
Language en
 
Identifier http://eprints.cmfri.org.in/11787/1/Genetic%20variation%20and%20phylogenetic%20relationship_AGKN%20Muneer%20et%20al.%20Mol%20Biol%20Rep%202011_38-4.pdf
Abdul Muneer, P M and Gopalakrishnan, A and Shivanandan, Remya and Basheer, V S and Ponniah, A G (2011) Genetic variation and phylogenetic relationship between two species of yellow catfish, Horabagrus brachysoma and H. nigricollaris (Teleostei: Horabagridae) based on RAPD and microsatellite markers. Molecular Biology Reports, 38 (4). pp. 2225-2232.