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DNA barcoding Indian marine fishes

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Relation http://eprints.cmfri.org.in/11792/
 
Title DNA barcoding Indian marine fishes
 
Creator Lakra, W S
Verma, M S
Goswami, Mukunda
Lal, Kuldeep Kumar
Mohindra, Vindhya
Punia, P
Gopalakrishnan, A
Singh, K V
Ward, Robert D
Hebert, P
 
Subject Fish Genetics
 
Description DNA barcoding has been adopted as a global bio-identification system for animals in recent years. A major national programme
on DNA barcoding of fish and marine life was initiated in India by the authors during 2006 and 115 species of
marine fish covering Carangids, Clupeids, Scombrids, Groupers, Sciaenids, Silverbellies, Mullids, Polynemids and Silurids
representing 79 Genera and 37 Families from the Indian Ocean have been barcoded for the first time using cytochrome
c oxidase I gene (COI) of the mtDNA. The species were represented by multiple specimens and a total of 397 sequences
were generated. After amplification and sequencing of 707 base pair fragment of COI, primers were trimmed which invariably
generated a 655 base pair barcode sequence. The average Kimura two parameter (K2P) distances within species, genera,
families, orders were 0.30%, 6.60%, 9.91%, 16.00%, respectively. In addition to barcode-based species identification
system, phylogenetic relationships among the species have also been attempted. The neighbour-joining tree revealed
distinct clusters in concurrence with the taxonomic status of the species.
 
Date 2011
 
Type Article
PeerReviewed
 
Format text
 
Language en
 
Identifier http://eprints.cmfri.org.in/11792/1/AGKN_2011_Molecular%20Ecology%20Resources_DNA%20barcoding%20Indian%20marine.pdf
Lakra, W S and Verma, M S and Goswami, Mukunda and Lal, Kuldeep Kumar and Mohindra, Vindhya and Punia, P and Gopalakrishnan, A and Singh, K V and Ward, Robert D and Hebert, P (2011) DNA barcoding Indian marine fishes. Molecular Ecology Resources, 11. pp. 60-71.