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Development and characterization of a fibroblastic-like cell line from caudal fin of the red-line torpedo, Puntius denisonii (Day) (Teleostei: Cyprinidae)

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Relation http://eprints.cmfri.org.in/11855/
http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2109.2011.02854.x/abstract
 
Title Development and characterization of a fibroblastic-like
cell line from caudal fin of the red-line torpedo, Puntius
denisonii (Day) (Teleostei: Cyprinidae)
 
Creator Raja Swaminathan, T
Lakra, W S
Gopalakrishnan, A
Basheer, V S
Kushwaha, B
Sajeela, K A
 
Subject Tropical fish
 
Description A fibroblastic-like cell line was established from the
ornamental ¢sh, red-line torpedo (Puntius denisonii).
The red-line torpedo fin (RTF) cell line is being maintained
in Leibovitz’s L-15 mediumsupplementedwith
10% fetal bovine serum (FBS) for over 1year at 28 1C
on a continuous basis in normal atmosphere. The
growth rate of RTF cells increased as the FBS proportion
increased from 5% to 20% at 28 1C with optimum
growth at the concentrations of 10% FBS. The
morphology of RTF cell was predominantly fibroblastic
like. Propagation of these cell lines was serum dependent,
with a low plating efficiency (o15%).
Karyotyping analysis of RTF cells at the 25th passage
indicated that the modal chromosome number was
2n550. The cell line was cryopreserved in liquid nitrogen
at �196 1C and could be recovered from storage
after 6 months with good cell viability.
Polymerase chain reaction amplification of a fragment
of two mitochondrial genes, 16S rRNA and
CO1, con¢rmed the identity of these cell lines with
those reported from this animal species, confirming
that the cell lines originated from P. denisonii. The
bacterial extracellular products from Vibrio cholerae
MTCC3904 and Aeromonas hydrophila were found to
be toxic to RTF. The cell lines were not susceptible to
viral nervous necrosis virus, a marine ¢sh virus.
 
Date 2012
 
Type Article
PeerReviewed
 
Format text
 
Language en
 
Identifier http://eprints.cmfri.org.in/11855/1/AGKN-2012_Aquaculture%20Research_Raja%20Swaminathan_Puntius%20denisonii.pdf
Raja Swaminathan, T and Lakra, W S and Gopalakrishnan, A and Basheer, V S and Kushwaha, B and Sajeela, K A (2012) Development and characterization of a fibroblastic-like cell line from caudal fin of the red-line torpedo, Puntius denisonii (Day) (Teleostei: Cyprinidae). Aquaculture Research, 43. pp. 498-508.