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Isolation, characterisation and phylogenetic diversity of culturable bacteria associated with marine microalgae from saline habitats of south India

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Relation http://eprints.cmfri.org.in/11871/
http://www.int-res.com/abstracts/ame/v79/n1/p21-30/
 
Title Isolation, characterisation and phylogenetic diversity
of culturable bacteria associated with marine
microalgae from saline habitats of south India
 
Creator Sandhya, S V
Preetha, K
Nair, V Anusree
Leo Antony, M
Vijayan, K K
 
Subject Bacteria
Algae
 
Description Cultivated microalgae are an essential source of nutrition to several farmed finfish,
shellfish and many other commercially significant aquaculture species. Knowledge of microalgaeassociated
microhabitat is important for the development of a successful, pathogen-free hatchery
rearing system. Therefore, an attempt was made to isolate (1), characterise (2) and determine the
phylogenetic diversity of (3) bacteria associated with cultured microalgae, which are used as live
feeds in many finfish and shellfish hatcheries. From 10 selected microalgal cultures, 34 bacterial
isolates were obtained with total bacterial counts of 101 to 105 CFU ml−1. Most notably, we checked
the presence of Vibrio spp., the major aquaculture pathogen in all tested microalgae and their
absence suggests the suitability of these microalgae for use in aquaculture systems. Phylogenetic
analysis based on 16S rDNA sequencing revealed that the bacterial phylotypes associated with
these microalgae were affiliated to Gammaproteobacteria, Alphaproteobacteria and Flavobacteriia
classes. The genus Marinobacter (47%) was found to be the most predominant cultivable
bacterium followed by Alteromonas, Labrenzia, Oceanicaulis, Ponticoccus, Stappia and Rheinheimera.
Bacteria belonging to the genera Gaetbulibacter and Maritalea were also detected and,
to the best of our knowledge, this is the first report of association of these bacterial groups with
microalgae. The biochemical, enzymatic and antibacterial characteristics and tolerance to various
abiotic stress factors of these bacterial isolates are also described in the present paper. Altogether,
the present study gives an insight into the phycosphere of cultivated microalgae, which can be
further explored for improving the productivity and reliability of indoor and outdoor microalgal
culture systems.
 
Date 2017
 
Type Article
PeerReviewed
 
Format text
 
Language en
 
Identifier http://eprints.cmfri.org.in/11871/1/Aquatic%20Microbial%20Ecology_2017_Sandhya%20S%20V_Isolation%2C%20characterisation%20and%20phylogenetic%20diversity.pdf
Sandhya, S V and Preetha, K and Nair, V Anusree and Leo Antony, M and Vijayan, K K (2017) Isolation, characterisation and phylogenetic diversity of culturable bacteria associated with marine microalgae from saline habitats of south India. Aquatic Microbial Ecology, 79 (1). pp. 21-30. ISSN 1616-1564