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Effect of extender composition on sperm cryopreservation of Asian catfish Heteropneustes fossilis (Bloch) and Clarias batrachus (Linnaeus)

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Relation http://eprints.cmfri.org.in/11933/
https://www.asianfisheriessociety.org/publication/archivedetails.php?id=24#
 
Title Effect of extender composition on sperm
cryopreservation of Asian catfish Heteropneustes
fossilis (Bloch) and Clarias batrachus (Linnaeus)
 
Creator Lal, Kuldeep Kumar
Barman, A S
Punia, P
Khare, Praveen
Mohindra, Vindhya
Lal, Bechan
Gopalakrishnan, A
Sah, Rama S
Lakra, W S
 
Subject Catfish
Fish Biotechnology
 
Description Air breathing catfish species Heteropneustes fossilis (Bloch) and Clarias batrachus
(Linn.) are important table fish and fetch high market price. Cryopreservation of spermatozoa
can be a useful tool in captive seed production for domestication and aquaculture of these catfish
species. The objective of the present study was to identify optimum extender composition for
sperm cryopreservation of the two species, H. fossilis and C. batrachus. Four extender
compositions Hank’s Balanced Salt Solution (HBSS), Modified Hank’s Balanced Salt Solution
(M-HBSS), Modified Hank’s Balanced Salt Solution with hen’s egg yolk (M-HBSS with EY)
and European catfish were evaluated for cryopreservation of catfish sperm and 10 % Dimethyle
Sulphoxide (DMSO) was used as a cryoprotectant. The pooled milt exhibiting 70-80% motile
sperm was used for cryopreservation experiment. After storage for 48 hrs at -196ºC, the milt was
thawed and evaluated for fertility test. The percentage of hatching was used as a parameter for
the comparative evaluation of different extender composition. In H. fossilis extender M-HBSS
indicated highest hatching rate (49.06%), followed by HBSS (42.76%), M-HBSS with EY
(37.46%) and European catfish (29.47%). The hatching success with extender M-HBSS did not
differ significantly (P > 0.05) from the control group (51%). In C. batrachus extender HBSS
exhibited highest hatching (62.1 %), followed by M-HBSS with EY (51.6%), European Catfish
(46.3%) and M-HBSS (40.9%). The hatching rate in control was 90% in C. batrachus. The
results indicated that the two species differ in the protocol for sperm cryopreservation. The
paper presents successful cryopreservation of sperm with the production of viable hatchlings of
H. fossilis and C. batrachus for the first time. The protocol reported in the study can be used for
scaling up of seed production of these two catfish species.
 
Publisher AFS Society
 
Date 2009
 
Type Article
PeerReviewed
 
Format text
 
Language en
 
Identifier http://eprints.cmfri.org.in/11933/1/AGKN_2009_Asian%20Fisheries%20Science_22_Cryopreservation%20Heteropneustes%20fossilis%20Sperm.pdf
Lal, Kuldeep Kumar and Barman, A S and Punia, P and Khare, Praveen and Mohindra, Vindhya and Lal, Bechan and Gopalakrishnan, A and Sah, Rama S and Lakra, W S (2009) Effect of extender composition on sperm cryopreservation of Asian catfish Heteropneustes fossilis (Bloch) and Clarias batrachus (Linnaeus). Asian Fisheries Science, 22. pp. 137-142.