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Growth performance of Spirulina (Arthrospira) platensis in a low cost medium: An assessment

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Relation http://eprints.cmfri.org.in/11956/
http://bioscipub.com/journals/abi/pdf/335-342.pdf
 
Title Growth performance of Spirulina (Arthrospira)
platensis in a low cost medium: An assessment
 
Creator Ranjith, L
Shukla, S P
Vennila, A
Purushothaman, C S
 
Subject Algae
 
Description The unialgal culture of Spirulina platensis was sub-cultured in Zarrouk’s medium under photoautotrophic
conditions. Initially, indoor batch cultivation was carried out for a week in four different types of cultivation
media viz., Zarrouk’s, Modified Zarrouk’s, prescribed Nallayam Research Centre (NRC), and Modified NRC.
In modified medium, urea and phosphoric acid of NRC medium were replaced with sodium nitrate and dipotassium
hydrogen phosphate (anhydrous) and concentration of ferrous sulphate heptahydrate was reduced.
The batch and airlift indoor culture experiments were carried out with an illumination of 3500±100 lux,
photoperiod of 12:12 hour light and dark periods and temperature of 24±1°C. The specific growth rate value
was 5.7 % higher in Zarrouk’s medium as compared to modified NRC medium. However, the cost of modified
NRC medium was considerably lower than Zarrouk’s medium; therefore, modified NRC medium was selected
for outdoor studies. The outdoor mass cultivation was done under natural conditions with the solar radiation
reaching the surface of culture was between 2160 and 8450 lux and temperature ranged from 27 to 34°C. An
assessment of the performance of growth in batch, airlift and FRP (Fiber Reinforced Polymer) tanks revealed
that culture grown in airlift units showed best growth which was evident from higher specific growth rate and
number of doublings per day. There was a 3.4-fold increase in cell density (in terms of turbidity at 750 nm) of
the cultures in such units. The growth in outdoor FRP tanks was also comparable to the airlift cultures.
 
Date 2013
 
Type Article
PeerReviewed
 
Format text
 
Language en
 
Identifier http://eprints.cmfri.org.in/11956/1/L.%20Ranjith%20%282013%29%20Growth%20%20%20performance%20of%20Spirulina%20%28Arthrospira%29%20platensis%20in%20a%20low%20cost%20medium%20An%20%20%20assessment.pdf
Ranjith, L and Shukla, S P and Vennila, A and Purushothaman, C S (2013) Growth performance of Spirulina (Arthrospira) platensis in a low cost medium: An assessment. Acta Biologica Indica, 2 (1). pp. 335-342.