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Involvement of plasmid in degradation of pentachlorophenol by Pseudomonoas sp. from a chemostat

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Title Involvement of plasmid in degradation of pentachlorophenol by Pseudomonoas sp. from a chemostat
 
Creator Thakur, Indu Shekhar
Verma, Praveen K.
Upadhaya, Kailash C.
 
Subject bacterial consortium
chemostat
cholohydroquinone
degradation
pentachlorophenol
plasmid
tetrachloro-p-hydroquinone
 
Description Pseudomonas sp. strain IST103 obtained from a stable bacterial consortium was capable of utilizing pentachlorophenol (PCP) as sole carbon and energy source. The consortium was developed by continuous enrichment in a chemostat. The degradation of PCP by bacterial strain proceeded through an oxidative route as indicated by accumulation of tetrachlorop-hydroquinone and chlorohydroquinone determined by high
performance liquid chromatography (HPLC), and
chloride molecules released in culture medium. Two
different molecular size plasmids, of approximately 80
and 4 kilobase, were found to be responsible for carrying genes for degradation of PCP. This was evidenced by mutants produced by curing of plasmid by
treatment of ethidium bromide. The derivatives were
not able to utilize PCP, however, transformation of low molecular size plasmid of Pseudomonas sp. strain 103 into E. coli JM109 utilized PCP, indicated a possible involvement of plasmid in degradation of pentachlorophenol
The experiment was partially supported by a research project of
Department of Biotechnology, Govt. of India, New Delhi. The author
(I.S.T.) thanks Department of Science and Technology, Govt. of India, for providing SERC-visiting Fellowship.
 
Date 2013-10-09T08:56:52Z
2013-10-09T08:56:52Z
2001
 
Type Article
 
Identifier Biochem. Biophys. Res. Comm., 286(1): 109-113
http://hdl.handle.net/123456789/19
 
Language en
 
Publisher Elsevier B.V.