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Level of endogenous free amino acids during various stages of culture of Vigna mungo (L.) Hepper - somatic embryogenesis, organogenesis and plant regeneration

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Title Level of endogenous free amino acids during various stages of culture of Vigna mungo (L.) Hepper - somatic embryogenesis, organogenesis and plant regeneration
 
Creator Sen, Jayanti
Kalia, Sanjeev
Guha-Mukherjee, Sipra
 
Subject Vigna mungo (L.) Hepper – Somatic embryogenesis
organogenesis and plant regeneration
 
Description A comparative study on endogenous free amino acid
levels was conducted in embryogenic and regenerating tissues vis-à-vis regenerated shoots of Vigna
mungo PS1. A number of important amino acids
were found to increase during initiation of embryogenesis and regeneration. To study the effects of exogenous application of amino acids in this system,
media were supplemented with three amino acids,
tryptophan (Trp), serine (Ser) and proline (Pro). We
found that in the presence of proline, a stress associated amino acid, there was an enhancement in
shoot-bud regeneration as well as in the production
of somatic embryos. We also analysed the endogenous levels of amino acids in these tissues. This communication thus deals with the induction of globular embryo by auxin and its promotion by
proline in V. mungo.
 
Date 2013-10-21T06:27:43Z
2013-10-21T06:27:43Z
2002
2002
 
Type Article
 
Identifier Current Science, 82: 429-433
http://hdl.handle.net/123456789/28
 
Language en
 
Publisher Indian Academy of Sciences