Record Details

The nuclear proteome of chickpea (Cicer arietinum L.) reveals predicted and unexpected proteins

NOPR - NISCAIR Online Periodicals Repository

View Archive Info
 
 
Field Value
 
Title The nuclear proteome of chickpea (Cicer arietinum L.) reveals predicted and unexpected proteins
 
Creator Pandey, Aarti
Choudhary, Mani Kant
Bhushan, Deepti
Chattopadhyay, Arnab
Chakraborty, Subhra
Datta, Asis
Chakraborty, Niranjan
 
Subject chickpea
nuclear proteins
proteome
2-DE
mass spectrometry
 
Description Nuclear proteins constitute a highly organized, complex network that plays diverse roles during cellular
development and other physiological processes. The yeast nuclear proteome corresponds to about
one-fourth of the total cellular proteins, suggesting the involvement of the nucleus in a number of
diverse functions. In an attempt to understand the complexity of plant nuclear proteins, we have
developed a proteome reference map of a legume, chickpea, using two-dimensional gel electrophoresis
(2-DE). Approximately, 600 protein spots were detected, and LC-ESI-MS/MS analyses led to the
identification of 150 proteins that have been implicated in a variety of cellular functions. The largest
percentage of the identified proteins was involved in signaling and gene regulation (36%), while 17%
were involved in DNA replication and transcription. The chickpea nuclear proteome indicates the
presence of few new nuclear proteins of unknown functions vis-a-vis many known resident proteins. To the best of our knowledge, this is the first report of a nuclear proteome of an unsequenced genome.
This work was supported by a
grant (BT/PR/4016/Agr/16/327) from the Department of
Biotechnology (DBT), Govt. of India, and a grant from National
Centre for Plant Genome Research, New Delhi, India, to N.C.
 
Date 2013-11-04T11:45:25Z
2013-11-04T11:45:25Z
2006
August 2006
 
Type Article
 
Identifier J. Proteome Res., 5(12): 3301-3311
http://hdl.handle.net/123456789/56
 
Language en
 
Publisher American Chemical Society