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Azadirachta indica leaf extract modulates initiation phase of murine forestomach tumorigenesis

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Title Azadirachta indica leaf extract modulates initiation phase of murine forestomach tumorigenesis
 
Creator Gangar, Subhash Chander
Koul, Ashwani
 
Subject Aryl hydrocarbon hydroxylase
Azadirachta indica
Benzo(a)pyrene
Lipid peroxidation
Tissue injury marker enzymes
UDP-glucuronosyltransferase
 
Description 209-215
The effects of aqueous Azadirachta indica leaf extract (AAILE) on benzo(a)pyrene [B(a)P]-induced forestomach tumorigenesis, B(a)P-DNA adduct formation and certain parameters of carcinogen biotransformation system in mice have been reported earlier from our laboratory. In this study, the effects of AAILE on the enzymes of B(a)P biotransformation, which play crucial role in initiation of chemical carcinogenesis  aryl hydrocarbon hydroxylase (AHH) and uridinediphosphoglucuronosyltransferase (UDP-glucuronosyltransferase) have been evaluated in murine forestomach and liver. In addition, lipid peroxidation (LPO) levels in forestomach as well as liver and the activities of tissue injury marker enzymes  lactate dehydrogenase, aspartate aminotransferase and alkaline phosphatase in the serum have also been evaluated. Oral administration of AAILE (100 mg/kg body wt for 2 weeks) reduces the AHH activity and enhances the UDP-glucuronosyltransferase activity in both the tissues, suggesting its potential in decreasing the activation and increasing the detoxification of carcinogens. The LPO levels decrease upon AAILE treatment in the hepatic tissue, suggesting its anti-oxidative and hence anti-carcinogenic effects. Non-significant alterations have been observed in tissue injury marker enzymes upon AAILE treatment, suggesting its safety at the given dose. In conclusion, AAILE appears to modulate initiation phase of carcinogenesis and may be suggested as safe and an effective agent for chemoprevention.
 
Date 2008-02-20T11:23:50Z
2008-02-20T11:23:50Z
2007-08
 
Type Article
 
Identifier 0301-1208
http://hdl.handle.net/123456789/117
 
Language en_US
 
Publisher CSIR
 
Source IJBB Vol.44(4) [August 2007]