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Metabolism of arachidonic acid in sheep uterus: In vitro studies

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Title Metabolism of arachidonic acid in sheep uterus: In vitro studies
 
Creator Padma, A Sai
Agarwal, S
Reddy, D Bharat
Prasad, T Sandeep
Reddanna, P
 
Subject Arachidonic acid metabosism
Lipoxygenase
Cyclooxygenase
Prostaglandins
HPETEs and HETEs
 
Description 216-222
Arachidonic acid (AA) metabolism in the non-pregnant sheep uterus was studied in vitro using conventional chromatographic and HPLC techniques. High expression of both lipoxygenase (LOX) as well as cyclooxygenase (COX) enzymes and their activities was found in the uterine tissues. On incubation of uterine enymes with AA, the LOX products formed were identified as 5-hydroperoxyeicosatetraenoic acid (5-HPETE), 12- and 15-hydroxyeicosatetraenoic acids
(12- and 15-HETEs), based on their separation on TLC and HPLC. By employing differential salt precipitation techniques, the LOXs generating products 5-HPETE (5-LOX), 12-HETE and 15-HETE (12- and 15-dual LOX) were isolated. Based on their analysis on TLC, the COX products formed were identified as prostaglandins - PGF2α and prostacyclin derivative 6-keto PGF1α. The study forms the first report on the comprehensive analysis on the metabolism of AA in sheep uterus in vitro via the LOX and COX pathways.
 
Date 2008-02-20T11:32:55Z
2008-02-20T11:32:55Z
2007-08
 
Type Article
 
Identifier 0301-1208
http://hdl.handle.net/123456789/118
 
Language en_US
 
Publisher CSIR
 
Source IJBB Vol.44(4) [August 2007]