Record Details


Field	Value

Title	Metabolism of arachidonic acid in sheep uterus: In vitro studies

Creator	Padma, A Sai Agarwal, S Reddy, D Bharat Prasad, T Sandeep Reddanna, P

Subject	Arachidonic acid metabosism Lipoxygenase Cyclooxygenase Prostaglandins HPETEs and HETEs

Description	216-222 Arachidonic acid (AA) metabolism in the non-pregnant sheep uterus was studied in vitro using conventional chromatographic and HPLC techniques. High expression of both lipoxygenase (LOX) as well as cyclooxygenase (COX) enzymes and their activities was found in the uterine tissues. On incubation of uterine enymes with AA, the LOX products formed were identified as 5-hydroperoxyeicosatetraenoic acid (5-HPETE), 12- and 15-hydroxyeicosatetraenoic acids (12- and 15-HETEs), based on their separation on TLC and HPLC. By employing differential salt precipitation techniques, the LOXs generating products 5-HPETE (5-LOX), 12-HETE and 15-HETE (12- and 15-dual LOX) were isolated. Based on their analysis on TLC, the COX products formed were identified as prostaglandins - PGF2α and prostacyclin derivative 6-keto PGF1α. The study forms the first report on the comprehensive analysis on the metabolism of AA in sheep uterus in vitro via the LOX and COX pathways.

Date	2008-02-20T11:32:55Z 2008-02-20T11:32:55Z 2007-08

Type	Article

Identifier	0301-1208 http://hdl.handle.net/123456789/118

Language	en_US

Publisher	CSIR

Source	IJBB Vol.44(4) [August 2007]

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