Record Details

Effect of water deficit on carbohydrate status and enzymes of carbohydrate metabolism in seedlings of wheat cultivars

NOPR - NISCAIR Online Periodicals Repository

View Archive Info
 
 
Field Value
 
Title Effect of water deficit on carbohydrate status and enzymes of carbohydrate metabolism in seedlings of wheat cultivars
 
Creator Kaur, Kamaljit
Gupta, Anil K
Kaur, Narinder
 
Subject Triticum aestivum L
Water deficit
Acid invertase
Alkaline invertase
⍺ and β-Amylase
Sucrose phosphate synthase
Sucrose synthase
Sucrose
 
Description 223-230
The effect of water deficit on carbohydrate status and enzymes of carbohydrate metabolism (⍺ and β amylases, sucrose phosphate synthase, sucrose synthase, acid and alkaline invertases) in wheat (Triticum aestivum L.) was investigated in the seedlings of drought-sensitive (PBW 343) and drought-tolerant (C 306) cultivars. The water deficit was induced by adding 6% mannitol (water potential -0.815 Mpa) in the growth medium. The water deficit reduced starch content in the shoots of tolerant seedlings as compared to the sensitive ones, but increased sucrose content in the shoots and roots of tolerant seedlings, indicating their protective role during stress conditions. It also decreased the ⍺-amylase activity in the endosperm of seedlings of both the cultivars, but increased ⍺ and β amylase activities in the shoots of tolerant ones. Sucrose phosphate synthase (SPS) activity showed a significant increase at 6 days of seedling growth (DSG) in the shoots of stressed seedlings of tolerant cultivar. However, SPS activity in the roots of stressed seedlings of sensitive cultivar was very low at 4 DSG and appeared significantly only at day 6. Sucrose synthase (SS) activity was lower in the shoots and roots of stressed seedlings of tolerant cultivar than sensitive ones at early stage of seedling growth. Higher acid invertase activity in the shoots of seedlings of tolerant cultivar appeared to be a unique characteristic of this cultivar for stress tolerance. Alkaline invertase activity, although affected under water deficit conditions, but was too low as compared to acid invertase activity to cause any significant affect on sucrose hydrolysis. In conclusion, higher sucrose content with high SPS and low acid invertase and SS activities in the roots under water deficit conditions could be responsible for drought tolerance of C 306.
 
Date 2008-02-20T11:49:09Z
2008-02-20T11:49:09Z
2007-08
 
Type Article
 
Identifier 0301-1208
http://hdl.handle.net/123456789/119
 
Language en_US
 
Publisher CSIR
 
Source IJBB Vol.44(4) [August 2007]