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Ion pairing RP-HPLC analytical methods for simultaneous estimation of simvastatin and its â-hydroxy acid

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Title Ion pairing RP-HPLC analytical methods for simultaneous estimation of simvastatin and its â-hydroxy acid
 
Creator Madan, Jitender
Thakkar, Vikrant
Dwivedi, Anil Kumar
Singh, Satyawan
 
Subject HPLC assay method
Hypolipidemic agent
Simvastatin
 
Description 371-376
Sensitive HPLC assay methods for Simvastatin (SV), a hypolipidemic agent, and its corresponding â-hydroxy
acid (SVA), were developed and validated for their simultaneous estimation in solutions of various studies. HPLC separations
were achieved on (i) C₈ Lichrocart® Lichrosphere® (ii) CN Lichrocart® Lichrosphere® and (iii) C₁₈ Phenomenex® columns.
The eluents were monitored by diode array detector at 240 nm. Retention times were: SV, 8-9; and SVA, 5.5-6 min. The
lower limit of detection of both SV and SVA on C₁₈ column was 0.05 μg/ml and on C₈ and CN columns was 0.1 μg/ml.
Reproducibility of the method were determined by inter and intra assay precision (< 6%).
 
Date 2008-05-06T11:24:20Z
2008-05-06T11:24:20Z
2007-05
 
Type Article
 
Identifier 0022-4456
http://hdl.handle.net/123456789/1252
 
Language en_US
 
Relation A61K
 
Publisher CSIR
 
Source JSIR Vol.66(5) [May 2007]